Anti-Nesprin3 antibody (ab190010)
Key features and details
- Rabbit polyclonal to Nesprin3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nesprin3 antibody
See all Nesprin3 primary antibodies -
Description
Rabbit polyclonal to Nesprin3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human Nesprin3 aa 150-250.
Database link: Q6ZM23 -
Positive control
- Human testis tissue; Jurkat cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS (without Mg2+, Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 112 kDa.
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IHC-P |
Use a concentration of 10 µg/ml.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 112 kDa. |
IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. -
Sequence similarities
Belongs to the nesprin family.
Contains 1 KASH domain.
Contains 2 spectrin repeats. -
Domain
The KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains. -
Cellular localization
Nucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells. - Information by UniProt
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Database links
- Entrez Gene: 161176 Human
- Entrez Gene: 212073 Mouse
- Omim: 610861 Human
- SwissProt: Q6ZMZ3 Human
- SwissProt: Q4FZC9 Mouse
- Unigene: 354645 Human
- Unigene: 212688 Mouse
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Alternative names
- C14orf49 antibody
- Chromosome 14 open reading frame 49 antibody
- FLJ25605 antibody
see all
Images
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All lanes : Anti-Nesprin3 antibody (ab190010) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate with synthesized peptide
Predicted band size: 112 kDa -
Immunohistochemical analysis of Formalin-Fixed, Paraffin-Embedded Human testis tissue labeling Nesprin3 with ab190010 at 10 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab190010 has not yet been referenced specifically in any publications.