Anti-Nesprin3 antibody (ab74261)
Key features and details
- Rabbit polyclonal to Nesprin3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Nesprin3 antibody
See all Nesprin3 primary antibodies -
Description
Rabbit polyclonal to Nesprin3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthesized peptide derived from an internal region of human Nesprin3.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Stabilizing agent. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitopespecific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 112 kDa.
|
Notes |
---|
WB
1/500 - 1/1000. Predicted molecular weight: 112 kDa. |
Target
-
Function
Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. -
Sequence similarities
Belongs to the nesprin family.
Contains 1 KASH domain.
Contains 2 spectrin repeats. -
Domain
The KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains. -
Cellular localization
Nucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells. - Information by UniProt
-
Database links
- Entrez Gene: 161176 Human
- Omim: 610861 Human
- SwissProt: Q6ZMZ3 Human
- Unigene: 354645 Human
-
Alternative names
- C14orf49 antibody
- Chromosome 14 open reading frame 49 antibody
- FLJ25605 antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab74261 has been referenced in 5 publications.
- Lee HP et al. The nuclear piston activates mechanosensitive ion channels to generate cell migration paths in confining microenvironments. Sci Adv 7:N/A (2021). PubMed: 33523987
- Mayca Pozo F et al. MYO10 drives genomic instability and inflammation in cancer. Sci Adv 7:eabg6908 (2021). PubMed: 34524844
- Sun RC et al. Nuclear Glycogenolysis Modulates Histone Acetylation in Human Non-Small Cell Lung Cancers. Cell Metab 30:903-916.e7 (2019). PubMed: 31523006
- Ji Y et al. Sperm-associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma. Oncol Rep 40:783-792 (2018). PubMed: 29901114
- Coffinier C et al. Abnormal development of the cerebral cortex and cerebellum in the setting of lamin B2 deficiency. Proc Natl Acad Sci U S A 107:5076-81 (2010). WB, ICC/IF ; Mouse . PubMed: 20145110