Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035)
Key features and details
- Mouse monoclonal [10C2] to Nestin - Neural Stem Cell Marker
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Nestin antibody [10C2] - Neural Stem Cell Marker
See all Nestin primary antibodies -
Description
Mouse monoclonal [10C2] to Nestin - Neural Stem Cell Marker -
Host species
Mouse -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Nestin aa 1-1621.
Database link: P48681 -
Positive control
- WB: U251 cells. ICC: U251 cells. Human brain tissue.
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General notes
Monoclonal antibody 10C2 was derived by fusion of P3x63Ag8.653 myeloma cells with spleen cells from a BALB/c mouse immunized with a 150-amino-acid fragment from the cloned human nestin, conjugated to glutathione S-transferase.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 59% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
10C2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 176 kDa.
1/1000 - 1/5000 using chemiluminescent detection. Predicted molecular weight: 176 kDa. |
Notes |
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ICC
Use at an assay dependent concentration. |
WB
1/1000 - 1/5000. Predicted molecular weight: 176 kDa. 1/1000 - 1/5000 using chemiluminescent detection. Predicted molecular weight: 176 kDa. |
Target
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Function
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. -
Tissue specificity
CNS stem cells. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments. -
Post-translational
modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. - Information by UniProt
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Database links
- Entrez Gene: 10763 Human
- Omim: 600915 Human
- SwissProt: P48681 Human
- Unigene: 527971 Human
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Alternative names
- ESTM 46 antibody
- FLJ 21841 antibody
- FLJ21841 antibody
see all
Images
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ab22035 staining human brain tissue cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 1% BSA for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the samples for 1 hour at 21°C. A FITC conjugated goat anti-mouse IgG antibody, diluted 1/250, was used as the secondary.
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ICC/IF of U251 cells staining intracellular Nestin filaments with ab22035
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Lane 1 : Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035) at 1/1000 dilution
Lane 2 : Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035)
Lane 1 : U251 cells at 10 µl
Lane 2 : U251 cells at 20 µl
Predicted band size: 176 kDa
Exposure time: 60 seconds -
Immunostaining of intracellular nestin filaments in cultured SH-SY-5Y neuronal cells using ab22035 (1:100 dilution).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (137)
ab22035 has been referenced in 137 publications.
- Tay SH et al. Generation of Cortical, Dopaminergic, Motor, and Sensory Neurons from Human Pluripotent Stem Cells. Methods Mol Biol 2549:359-377 (2022). PubMed: 33959917
- Chen J et al. Proteomic Distributions in CD34+ Microvascular Niche Patterns of Glioblastoma. J Histochem Cytochem 70:99-110 (2022). PubMed: 34751042
- Koessinger AL et al. Increased apoptotic sensitivity of glioblastoma enables therapeutic targeting by BH3-mimetics. Cell Death Differ 29:2089-2104 (2022). PubMed: 35473984
- Papes F et al. Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content. Nat Commun 13:2387 (2022). PubMed: 35501322
- Brezovakova V et al. Astrocytes Derived from Familial and Sporadic Alzheimer's Disease iPSCs Show Altered Calcium Signaling and Respond Differently to Misfolded Protein Tau. Cells 11:N/A (2022). PubMed: 35563735