• Product nameAnti-Nestin antibody [10C2]See all Nestin primary antibodies ...
  • Description
    Mouse monoclonal [10C2] to Nestin
  • SpecificityReacts exclusively with human nestin protein.
  • Tested applicationsICC/IF, IHC-Fr, IHC-P, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A recombinant 150 amino acid fragment from human Nestin conjugated to glutathione S transferase.

  • General notesOnce thawed refrigerate. Do not refreeze.



Our Abpromise guarantee covers the use of ab22035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF 1/1000. PubMed: 191475021/1000.
IHC-Fr 1/100 - 1/200.
IHC-P Use at an assay dependent dilution.
WB 1/500 - 1/2500. Predicted molecular weight: 176 kDa. 1/1000 - 1/5000 using chemiluminescent detection. Predicted molecular weight: 176 kDa.


  • FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • Tissue specificityCNS stem cells.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • Post-translational
    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Target information above from: UniProt accession P48681 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • Nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

Anti-Nestin antibody [10C2] images

  • ab22035 staining human brain tissue cells by ICC/IF.  Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 1% BSA for 30 minutes at 21°C.  The primary antibody was diluted 1/100 and incubated with the samples for 1 hour at 21°C.  A FITC conjugated goat anti-mouse IgG antibody, diluted 1/250, was used as the secondary.

    See Abreview

  • Lane 1 : Anti-Nestin antibody [10C2] (ab22035) at 1/1000 dilution
    Lane 2 : Anti-Nestin antibody [10C2] (ab22035)

    Lane 1 : U251 cells at 10 µl
    Lane 2 : U251 cells at 20 µl

    Predicted band size : 176 kDa

    Exposure time : 60 seconds

References for Anti-Nestin antibody [10C2] (ab22035)

This product has been referenced in:
  • Bignone PA  et al. Identification of human embryonic progenitor cell targeting peptides using phage display. PLoS One 8:e58200 (2013). ICC/IF ; Human . Read more (PubMed: 23483995) »
  • Long PM  et al. N-acetylaspartate (NAA) and N-acetylaspartylglutamate (NAAG) promote growth and inhibit differentiation of glioma stem-like cells. J Biol Chem 288:26188-200 (2013). Read more (PubMed: 23884408) »

See all 11 Publications for this product

Product Wall

Thank you for contacting us.

1. Anti-Nestin antibody [10C2] (ab22035) and Anti-HIF1 alpha antibody [H1alpha67] - BSA and Azide free (ab80544):

Volume: 100 µg at 1 mg/ml

We recommend to start with a concentration of 5 u...

Read More

Thank you very much for your interest in anti-Nestin antibodies.

To our knowledge our anti-Nestin products have not been tested in bovine. Therefore, I can offer a discount off a future purchase if you buy an anti-Nestin antibody now, test it...

Read More

Thank you very much for your email. I apologize for the delay in our answer.

I can confirm thatthis antibody can be used with HRP conjugated secondary antibody without any problems.

The note on the datasheet is in reference on how i...

Read More
Application Flow Cytometry
Sample Human Cell (Differentiation Human ES cell)
Specification Differentiation Human ES cell
Preparation Cell harvesting/tissue preparation method: Cell dissociation buffer
Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
Fixation Formaldehyde
Permeabilization Yes - 70% methanol (ice cold)
Gating Strategy FSC vs SSC on negative control sample

Abcam user community

Verified customer

Submitted Jan 09 2012

Thank you for your patience. I have attached the test results from the lab. We performed an immunoblotting experiment on a cell extract of U251 cells. The attached Western blot image clearly indicates a Nestin band with a MW of approximately 250 kDa. T...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (brain tumor cell line)
Specification brain tumor cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 29 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human ES cell derived neurons)
Specification human ES cell derived neurons
Fixative Paraformaldehyde
Permeabilization Yes - See Blocking buffer
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jun 21 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (brain tissues)
Specification brain tissues
Fixative Paraformaldehyde
Permeabilization Yes - triton-x
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C

Dr. Amal Shervington

Verified customer

Submitted Jul 25 2008

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Acetone
Permeabilization Yes - 0.1% Triton X-100
Blocking step Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 23 2008

The tissue used to validate the nestin antibody for IHC on paraffin-embedded samples is human fetal brains. Development of the antibody is described in the following reference: Messam CA et al. Coexpression of nestin in neural and glial cells in th...

Read More