Overview

  • Product nameAnti-Nestin antibody [10C2]
    See all Nestin primary antibodies
  • Description
    Mouse monoclonal [10C2] to Nestin
  • SpecificityReacts exclusively with human nestin protein.
  • Tested applicationsICC/IF, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A recombinant 150 amino acid fragment from human Nestin conjugated to glutathione S transferase.

  • General notesOnce thawed refrigerate. Do not refreeze.

Properties

Applications

Our Abpromise guarantee covers the use of ab22035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/200. PubMed: 19147502

1/1000.

IHC-Fr 1/100 - 1/200.
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Predicted molecular weight: 176 kDa.

1/1000 - 1/5000 using chemiluminescent detection. Predicted molecular weight: 176 kDa.

Target

  • FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • Tissue specificityCNS stem cells.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • Post-translational
    modifications
    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Information by UniProt
  • Database links
  • Alternative names
    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • Nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

Anti-Nestin antibody [10C2] images

  • ab22035 staining human brain tissue cells by ICC/IF.  Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 1% BSA for 30 minutes at 21°C.  The primary antibody was diluted 1/100 and incubated with the samples for 1 hour at 21°C.  A FITC conjugated goat anti-mouse IgG antibody, diluted 1/250, was used as the secondary.

    See Abreview

  • Lane 1 : Anti-Nestin antibody [10C2] (ab22035) at 1/1000 dilution
    Lane 2 : Anti-Nestin antibody [10C2] (ab22035)

    Lane 1 : U251 cells at 10 µl
    Lane 2 : U251 cells at 20 µl


    Predicted band size : 176 kDa


    Exposure time : 60 seconds

References for Anti-Nestin antibody [10C2] (ab22035)

This product has been referenced in:
  • Berardis S  et al. Gene expression profiling and secretome analysis differentiate adult-derived human liver stem/progenitor cells and human hepatic stellate cells. PLoS One 9:e86137 (2014). IHC-P ; Human . Read more (PubMed: 24516514) »
  • Macarthur KM  et al. Detection of brain tumor cells in the peripheral blood by a telomerase promoter-based assay. Cancer Res 74:2152-9 (2014). ICC/IF ; Human . Read more (PubMed: 24525740) »

See all 14 Publications for this product

Product Wall

Thank you for contacting us.

1. Anti-Nestin antibody [10C2] (ab22035) and Anti-HIF1 alpha antibody [H1alpha67] - BSA and Azide free (ab80544):

Volume: 100 µg at 1 mg/ml

We recommend to start with a concentration of 5 u...

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Thank you very much for your interest in anti-Nestin antibodies.

To our knowledge our anti-Nestin products have not been tested in bovine. Therefore, I can offer a discount off a future purchase if you buy an anti-Nestin antibody now, test it...

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Thank you very much for your email. I apologize for the delay in our answer.

I can confirm thatthis antibody can be used with HRP conjugated secondary antibody without any problems.

The note on the datasheet is in reference on how i...

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Application Flow Cytometry
Sample Human Cell (Differentiation Human ES cell)
Specification Differentiation Human ES cell
Preparation Cell harvesting/tissue preparation method: Cell dissociation buffer
Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
Fixation Formaldehyde
Permeabilization Yes - 70% methanol (ice cold)
Gating Strategy FSC vs SSC on negative control sample
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Abcam user community

Verified customer

Submitted Jan 09 2012

Thank you for your patience. I have attached the test results from the lab. We performed an immunoblotting experiment on a cell extract of U251 cells. The attached Western blot image clearly indicates a Nestin band with a MW of approximately 250 kDa. T...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (brain tumor cell line)
Specification brain tumor cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 29 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human ES cell derived neurons)
Specification human ES cell derived neurons
Fixative Paraformaldehyde
Permeabilization Yes - See Blocking buffer
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jun 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (brain tissues)
Specification brain tissues
Fixative Paraformaldehyde
Permeabilization Yes - triton-x
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Dr. Amal Shervington

Verified customer

Submitted Jul 25 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Acetone
Permeabilization Yes - 0.1% Triton X-100
Blocking step Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 23 2008

The tissue used to validate the nestin antibody for IHC on paraffin-embedded samples is human fetal brains. Development of the antibody is described in the following reference: Messam CA et al. Coexpression of nestin in neural and glial cells in th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"