Overview
- Product nameAnti-Nestin antibody [10C2]See all Nestin primary antibodies ...
- DescriptionMouse monoclonal [10C2] to Nestin
- SpecificityReacts exclusively with human nestin protein.
- Tested applicationsICC/IF, IHC-Fr, IHC-P, WB more details
- Species reactivityReacts with: Human
- Immunogen
A recombinant 150 amino acid fragment from human Nestin conjugated to glutathione S transferase.
- General notesOnce thawed refrigerate. Do not refreeze.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityProtein G purified
- Clonality Monoclonal
- Clone number10C2
- IsotypeIgG1
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab22035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: 1/1000. PubMed: 191475021/1000. |
| IHC-Fr | IHC-Fr: 1/100 - 1/200. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| WB | WB: 1/500 - 1/2500. Predicted molecular weight: 176 kDa. 1/1000 - 1/5000 using chemiluminescent detection. Predicted molecular weight: 176 kDa. |
Target
- FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
- Tissue specificityCNS stem cells.
- Sequence similaritiesBelongs to the intermediate filament family.
- Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
- Post-translational
modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. -
Database links
- Entrez Gene: 10763 Human
- Omim: 600915 Human
- SwissProt: P48681 Human
- Unigene: 527971 Human
Target information above from: UniProt accession
P48681
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ESTM 46 antibodyFLJ 21841 antibodyFLJ21841 antibody
- Intermediate filament protein antibodyNbla00170 antibodyNes antibodyNEST_HUMAN antibodyNestin antibody
see all
Anti-Nestin antibody [10C2] images
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![Immunocytochemistry/ Immunofluorescence - Nestin antibody [10C2] (ab22035)](http://a.abcam.com/ps/datasheet/Images/22/ab22035/ab22035_3.jpg)
Immunocytochemistry/ Immunofluorescence - Nestin antibody [10C2] (ab22035)This image is courtesy of an Abreview submitted by Dr Amal Shervingtonab22035 staining human brain tissue cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 1% BSA for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the samples for 1 hour at 21°C. A FITC conjugated goat anti-mouse IgG antibody, diluted 1/250, was used as the secondary.
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![Western blot - Anti-Nestin antibody [10C2] (ab22035)](http://a.abcam.com/ps/datasheet/images/22/ab22035/Nestin-Primary-antibodies-ab22035-17.jpg)
Western blot - Anti-Nestin antibody [10C2] (ab22035)Lane 1 : Anti-Nestin antibody [10C2] (ab22035) at 1/1000 dilution
Lane 2 : Anti-Nestin antibody [10C2] (ab22035)
Lane 1 : U251 cells at 10 µl
Lane 2 : U251 cells at 20 µl
Predicted band size : 176 kDa
Exposure time : 60 seconds
References for Anti-Nestin antibody [10C2] (ab22035)
This product has been referenced in:
- Xu DS et al. Neuronatin in a subset of glioblastoma multiforme tumor progenitor cells is associated with increased cell proliferation and shorter patient survival. PLoS One 7:e37811 (2012). WB . Read more (PubMed: 22624064) »
- Jouhilahti EM et al. The development of cutaneous neurofibromas. Am J Pathol 178:500-5 (2011). IHC-P ; Human . Read more (PubMed: 21281783) »
