Anti-Nestin antibody [401] - Neural Stem Cell Marker (ab11306)

Overview

• Product nameAnti-Nestin antibody [401] - Neural Stem Cell MarkerSee all Nestin primary antibodies ...
• Description
  Mouse monoclonal [401] to Nestin - Neural Stem Cell Marker
• Tested applicationsICC/IF, ICC, IHC-Fr, WB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat
  

  Does not react with

  Human
• Immunogen

  Nestin purified from embryonic rat spinal cord.

• Positive controlMEF1 whole cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS, pH 7.4
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone number401
• MyelomaNS1
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Developmental Biology
  • Lineage specification
  • Ectoderm

  • Stem Cells
  • Neural Stem Cells
  • Intracellular

  • Stem Cells
  • Neural Stem Cells
  • Neuron Restricted Lineage

  • Stem Cells
  • Lineage Markers
  • Ectoderm

  • Cancer
  • Invasion/microenvironment
  • Angiogenesis
  • Other

  • Cancer
  • Tumor biomarkers
  • Tumor antigens

  • Cardiovascular
  • Angiogenesis
  • Angiogenic Factors

  • Neuroscience
  • Cell Adhesion Proteins
  • Cytoskeletal Proteins
  • Intermediate Filaments

  • Neuroscience
  • Neurology process
  • Neurogenesis

  • Neuroscience
  • Cell Type Marker
  • Neural Stem Cell marker

Applications

Target

• FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
• Tissue specificityCNS stem cells.
• Sequence similaritiesBelongs to the intermediate filament family.
• Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
• Post-translational
  modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Target information above from: UniProt accession P48681 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 18008 Mouse
  • Entrez Gene: 25491 Rat
  • SwissProt: Q6P5H2 Mouse
  • SwissProt: P21263 Rat
  • Unigene: 331129 Mouse
  • Unigene: 9701 Rat

• Alternative names
  ESTM 46 antibodyESTM 46 antibodyFLJ 21841 antibody

  FLJ 21841 antibodyFLJ21841 antibodyIntermediate filament protein antibodyIntermediate filament protein antibodyNbla00170 antibodyNes antibodyNes antibodyNEST_HUMAN antibodyNestin antibody

  see all

Anti-Nestin antibody [401] - Neural Stem Cell Marker images

• 

  Western blot - Anti-Nestin antibody [401] - Neural Stem Cell Marker (ab11306)
  Anti-Nestin antibody [401] - Neural Stem Cell Marker (ab11306) at 1 µg/ml + MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
  
  Secondary
  Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 177 kDa
  
  
  Exposure time : 20 minutes
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nestin antibody [401] - Neural Stem Cell Marker (ab11306)This image is courtesy of an Abreview submitted by Mr Carl Hobbs

  ab11306 staining Nestin in rat lateral ventricle (subventricular zone)  sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with  1% BSA for 10 minutes at room temperature. Antigen retrieval was by heat mediation in citric acid (pH6). Samples were incubated with primary antibody 1/200 (TBS/BSA/azide pH7.5) for 24 hours. A Biotin-conjugated Goat polycolonal to mouse IgG, dilution 1/200, was used as secondary antibody. The image shows clear staining in cells of the rat subventricular zone of the lateral ventricle.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nestin antibody [401] - Neural Stem Cell Marker (ab11306)
  IHC image of Nestin staining in 6 week old rat brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11306, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
• 

  Immunocytochemistry/ Immunofluorescence - Nestin antibody [401] - Neural Stem Cell Marker (ab11306)
  ICC/IF image of ab11306 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11306, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 10µg/ml.