Anti-Nestin antibody [7A3] (ab81462)

Overview

  • Product nameAnti-Nestin antibody [7A3]See all Nestin primary antibodies ...
  • Description
    Rat monoclonal [7A3] to Nestin
  • Tested applicationsIHC-Fr, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse E16 embryonic cerebral cortex extracts

  • Positive control
    • Neural progenitor cells from mouse fetal brain.
  • General notes


    ab81462 is very useful for immunostaining of mouse embryonic brain. It has very low background in immunostaining using mouse tissues and is also useful for doubleb staining with mouse and rabbit antibodies.

Properties

Applications

Our Abpromise guarantee covers the use of ab81462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-Fr Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • Tissue specificityCNS stem cells.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • Post-translational
      modifications
      Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Target information above from: UniProt accession P48681 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • Nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    Anti-Nestin antibody [7A3] images

    • Primary culture of neural progenitor cells from mouse fetal brain stained with ab81462.
    • Primary culture of neural progenitor cells from mouse fetal brain, without staining (bright field image).

    References for Anti-Nestin antibody [7A3] (ab81462)

    ab81462 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Cor1 cells)
    Specification Cor1 cells
    Fixative Formaldehyde
    Permeabilization Yes - 0.1% TWEEN
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted May 25 2011

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Brain and kidney)
    Specification Brain and kidney
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 13 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"