Overview
- Product nameAnti-Nestin antibody [7A3]See all Nestin primary antibodies ...
- DescriptionRat monoclonal [7A3] to Nestin
- Tested applicationsIHC-Fr, ICC/IF more details
- Species reactivityReacts with: Mouse
- Immunogen
Mouse E16 embryonic cerebral cortex extracts
- Positive controlNeural progenitor cells from mouse fetal brain.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 50% Glycerol, PBS -
Concentration information loading... - PurityIgG fraction
- Purification notesThe antibody was produced from the hybridoma cultured in serum free medium and purified under mild conditions by propriety chromatography processes. It is filter sterilized.
- Primary antibody notes ab81462 is very useful for immunostaining of mouse embryonic brain. It has very low background in immunostaining using mouse tissues and is also useful for doubleb staining with mouse and rabbit antibodies.
- Clonality Monoclonal
- Clone number7A3
- IsotypeIgG2b
- Light chain typekappa
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab81462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-Fr | IHC-Fr: Use at an assay dependent dilution. |
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
Target
- FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
- Tissue specificityCNS stem cells.
- Sequence similaritiesBelongs to the intermediate filament family.
- Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
- Post-translational
modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. -
Database links
- Entrez Gene: 18008 Mouse
- SwissProt: Q6P5H2 Mouse
- Unigene: 331129 Mouse
Target information above from: UniProt accession
P48681
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- ESTM 46 antibodyFLJ 21841 antibodyFLJ21841 antibody
- Intermediate filament protein antibodyNbla00170 antibodyNes antibodyNEST_HUMAN antibodyNestin antibody
see all
Anti-Nestin antibody [7A3] images
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![Immunocytochemistry/ Immunofluorescence - Nestin antibody [7A3] (ab81462)](http://a.abcam.com/ps/datasheet/images/81/ab81462/Nestin-Primary-antibodies-ab81462-9.jpg)
Immunocytochemistry/ Immunofluorescence - Nestin antibody [7A3] (ab81462)Primary culture of neural progenitor cells from mouse fetal brain stained with ab81462. -
![Immunocytochemistry/ Immunofluorescence - Nestin antibody [7A3] (ab81462)](http://a.abcam.com/ps/datasheet/images/81/ab81462/Nestin-Primary-antibodies-ab81462-8.jpg)
Immunocytochemistry/ Immunofluorescence - Nestin antibody [7A3] (ab81462)Primary culture of neural progenitor cells from mouse fetal brain, without staining (bright field image).
References for Anti-Nestin antibody [7A3] (ab81462)
ab81462 has not yet been referenced specifically in any publications.
