Anti-Nestin antibody - Aminoterminal end (ab76684)

Overview

• Product nameAnti-Nestin antibody - Aminoterminal endSee all Nestin primary antibodies ...
• Description
  Rabbit polyclonal to Nestin - Aminoterminal end
• Tested applicationsIHC-P, ELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide selected from the N-terminal region of human Nestin conjugated to KLH

• Positive controlHuman breast carcinoma tissue.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesThis antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Lineage specification
  • Ectoderm

  • Stem Cells
  • Neural Stem Cells
  • Neuron Restricted Lineage

  • Stem Cells
  • Neural Stem Cells
  • Intracellular

  • Stem Cells
  • Lineage Markers
  • Ectoderm

  • Cancer
  • Invasion/microenvironment
  • Angiogenesis
  • Other

  • Cancer
  • Tumor biomarkers
  • Tumor antigens

  • Cardiovascular
  • Angiogenesis
  • Angiogenic Factors

  • Neuroscience
  • Neurology process
  • Neurogenesis

  • Neuroscience
  • Cell Type Marker
  • Neural Stem Cell marker

  • Neuroscience
  • Cell Adhesion Proteins
  • Cytoskeletal Proteins
  • Intermediate Filaments

Applications

Target

• FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
• Tissue specificityCNS stem cells.
• Sequence similaritiesBelongs to the intermediate filament family.
• Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
• Post-translational
  modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Target information above from: UniProt accession P48681 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10763 Human
  • Omim: 600915 Human
  • SwissProt: P48681 Human
  • Unigene: 527971 Human

• Alternative names
  ESTM 46 antibodyFLJ 21841 antibodyFLJ21841 antibody

  Intermediate filament protein antibodyNbla00170 antibodyNes antibodyNEST_HUMAN antibodyNestin antibody

  see all

Anti-Nestin antibody - Aminoterminal end images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nestin antibody (ab76684)
  ab76684, at a 1/50 dilution, staining Nestin in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry. A peroxidase conjugated secondary antibody was then used, followed by DAB staining.