Anti-Nestin antibody - Azide free (ab27952)

Overview

  • Product nameAnti-Nestin antibody - Azide freeSee all Nestin primary antibodies ...
  • Description
    Rabbit polyclonal to Nestin - Azide free
  • SpecificityAb27952 recognises nestin tail.
  • Tested applicationsWB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Apteronotus leptorhynchus
  • Immunogen

    KLH conjugated peptide to the human nestin tail.

  • Positive control
    • Human NTERA2 cells and mouse embryonic cells for Immunofluorescence.

Applications

Our Abpromise guarantee covers the use of ab27952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 177 kDa.
IHC-Fr Use at an assay dependent concentration.

IHC-Fr is not batch-tested and variability between batches can occur.

ICC/IF 1/200.

Target

  • FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • Tissue specificityCNS stem cells.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • Post-translational
    modifications
    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Information by UniProt
  • Database links
  • Alternative names
    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • Nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

Anti-Nestin antibody - Azide free images

  • ab27952 staining rat astrocytes by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Saponin prior to blocking in 10% serum for 1 hour at 20°C.  The primary antibody was diluted 1/500 and incubated with the sample for 18 hours at 4°C.  A Cy3® conjugated goat anti-rabbit IgG antibody diluted 1/400 was used as the secondary.

    See Abreview

  • ab27952 staining Nestin in Apteronotus leptorynchus spinal cord tissue by Immunohistochemistry (Frozen sections). The sections were fixed in 2% paraformaldehye and permeabilized in 0.3% Triton X-100 prior to blocking with 3% sheep serum / 1% BSA / 1% teleostean gelatine in PBS for 1 hour at 23°C. The primary antibody was diluted 1/100 in the blocking solution and incubated with the sample for 18 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

  • ab27952 staining Nestin in human embryonic stem cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using Triton-X, blocked with 5% serum for 2 hours at 25°C and then incubated with ab27952 at a 1/100 dilution for 12 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey polyclonal used at a 1/500 dilution.

    See Abreview

References for Anti-Nestin antibody - Azide free (ab27952)

This product has been referenced in:
  • Sandstrom RS  et al. Epigenetic Regulation by Chromatin Activation Mark H3K4me3 in Primate Progenitor Cells within Adult Neurogenic Niche. Sci Rep 4:5371 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24947819) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Dorsal Root Ganglia (DRG) neurons)
Specification Dorsal Root Ganglia (DRG) neurons
Fixative Paraformaldehyde
Permeabilization Yes - Tween 20
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Oct 22 2012

Thank you very much for your interest in anti-Nestin antibodies.

To our knowledge our anti-Nestin products have not been tested in bovine. Therefore, I can offer a discount off a future purchase if you buy an anti-Nestin antibody now, test it...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Embryonic Stem Cells)
Specification Embryonic Stem Cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton-X
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 29 2012

DISCOUNT CODE: xx
Ablaufdatum: dd.mm.2012

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab27952 in der IHC-P zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weitere...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Brain, Spinal cord)
Specification Brain, Spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Sep 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (freshly dispersed myocytes from mesenteric artery)
Specification freshly dispersed myocytes from mesenteric artery
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (neural stem cell line)
Specification neural stem cell line
Fixative Formaldehyde
Permeabilization Yes - 5 minutes 0.2% Tween-20
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Apr 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (astrocytes)
Specification astrocytes
Fixative Paraformaldehyde
Permeabilization Yes - 0,5% Saponin
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Mr. Heinrich Bürgers

Verified customer

Submitted Feb 22 2008

Thank you for your enquiry. Ab27952 is a rabbit polyclonal antibody to nestin, and ab6320 is a mouse monoclonal antibody. The immunogen used to generate ab27952 was a KLH conjugated peptide to the human nestin tail. The immunogen used to generate ab632...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"