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KLH conjugated peptide to the human nestin tail.
Our Abpromise guarantee covers the use of ab27952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 177 kDa.|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.
IHC-Fr is not batch-tested and variability between batches can occur.
ab27952 staining rat astrocytes by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Saponin prior to blocking in 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/500 and incubated with the sample for 18 hours at 4°C. A Cy3® conjugated goat anti-rabbit IgG antibody diluted 1/400 was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"