Overview

  • Product nameAnti-Nestin antibody - Azide freeSee all Nestin primary antibodies ...
  • Description
    Rabbit polyclonal to Nestin - Azide free
  • Tested applicationsWB, ICC/IF, IHC-P more details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse
  • Immunogen

    KLH conjugated peptide to the human nestin repeat region.

  • Positive control
    • Ntera 2 cells
  • General notes


    ab28944 can be used as a predominant marker to describe stem and neural progenitor cells in the mammalian CNS.

Properties

Applications

Our Abpromise guarantee covers the use of ab28944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000. Predicted molecular weight: 177 kDa.
ICC/IF 1/200.
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • Tissue specificityCNS stem cells.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • Post-translational
    modifications
    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Target information above from: UniProt accession P48681 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • Nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

Anti-Nestin antibody - Azide free images

  • Immunofluorescence analysis of Nestin expression in methanol fixed Ntera2 cells stained using 1:200 anti nestin (ab28944, green). The sample was counterstained with Vimentin (red). The micrograph with yellow staining shows overlapping of nestin and vimentin in the cells. A light phase micrograph shows the same cell as a whole.

References for Anti-Nestin antibody - Azide free (ab28944)

This product has been referenced in:
  • Wang Z  et al. Acquisition of epithelial-mesenchymal transition phenotype of gemcitabine-resistant pancreatic cancer cells is linked with activation of the notch signaling pathway. Cancer Res 69:2400-7 (2009). WB ; Human . Read more (PubMed: 19276344) »
  • Pereira CF  et al. Heterokaryon-based reprogramming of human B lymphocytes for pluripotency requires Oct4 but not Sox2. PLoS Genet 4:e1000170 (2008). ICC/IF ; Human . Read more (PubMed: 18773085) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric buffer, pH6.0
Sample Human Tissue sections (adult skin)
Specification adult skin
Permeabilization Yes - Tween-20
Fixative Paraformaldehyde
Username

Mr. Heiko Locher

Verified customer

Submitted Sep 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"