Anti-Nestin antibody - Azide free (ab28944)
Overview
- Product nameAnti-Nestin antibody - Azide freeSee all Nestin primary antibodies ...
- DescriptionRabbit polyclonal to Nestin - Azide free
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Human
Does not react with
Mouse - Immunogen
KLH conjugated peptide to the human nestin repeat region.
- Positive controlNtera 2 cells
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: Whole serum - PurityWhole antiserum
- Purification notesPurified using a desalting column.
- Primary antibody notes ab28944 can be used as a predominant marker to describe stem and neural progenitor cells in the mammalian CNS.
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab28944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000. Predicted molecular weight: 177 kDa. |
| ICC/IF | ICC/IF: 1/200. |
Target
- FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
- Tissue specificityCNS stem cells.
- Sequence similaritiesBelongs to the intermediate filament family.
- Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
- Post-translational
modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. -
Database links
- Entrez Gene: 10763 Human
- Omim: 600915 Human
- SwissProt: P48681 Human
- Unigene: 527971 Human
Target information above from: UniProt accession
P48681
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ESTM 46 antibodyFLJ 21841 antibodyFLJ21841 antibody
- Intermediate filament protein antibodyNbla00170 antibodyNes antibodyNEST_HUMAN antibodyNestin antibody
see all
Anti-Nestin antibody - Azide free images
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Immunocytochemistry/ Immunofluorescence - Nestin antibody (ab28944)Immunofluorescence analysis of Nestin expression in methanol fixed Ntera2 cells stained using 1:200 anti nestin (ab28944, green). The sample was counterstained with Vimentin (red). The micrograph with yellow staining shows overlapping of nestin and vimentin in the cells. A light phase micrograph shows the same cell as a whole.
References for Anti-Nestin antibody - Azide free (ab28944)
This product has been referenced in:
- Wang Z et al. Acquisition of epithelial-mesenchymal transition phenotype of gemcitabine-resistant pancreatic cancer cells is linked with activation of the notch signaling pathway. Cancer Res 69:2400-7 (2009). WB ; Human . Read more (PubMed: 19276344) »
- Pereira CF et al. Heterokaryon-based reprogramming of human B lymphocytes for pluripotency requires Oct4 but not Sox2. PLoS Genet 4:e1000170 (2008). ICC/IF ; Human . Read more (PubMed: 18773085) »
