Directly conjugated antibodies for immunofluorescence

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Anti-Nestin antibody - Neural Stem Cell Marker (ab24692)


  • Product nameAnti-Nestin antibody - Neural Stem Cell Marker
    See all Nestin primary antibodies
  • Description
    Rabbit polyclonal to Nestin - Neural Stem Cell Marker
  • Tested applicationsIHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse

    Does not react with

  • Immunogen

    Synthetic peptide of Mouse Nestin repeat sequence conjugated to KLH. Immunogen sequence is proprietary

  • Positive control
    • Mouse embryonic cells.


Our Abpromise guarantee covers the use of ab24692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution. PubMed: 17389499
WB 1/1000. Predicted molecular weight: 220-240 kDa.
ICC/IF 1/200.


  • FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • Tissue specificityCNS stem cells.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • Post-translational
    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Information by UniProt
  • Database links
  • Alternative names
    • ESTM 46 antibody
    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • Nes antibody
    • Nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

References for Anti-Nestin antibody - Neural Stem Cell Marker (ab24692)

This product has been referenced in:
  • Belsham DD  et al. Ciliary neurotrophic factor recruitment of glucagon-like peptide-1 mediates neurogenesis, allowing immortalization of adult murine hypothalamic neurons. FASEB J 23:4256-65 (2009). ICC/IF ; Mouse . Read more (PubMed: 19703933) »
  • Xu H  et al. Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes. Invest Ophthalmol Vis Sci 48:1674-82 (2007). IHC-Fr ; Mouse . Read more (PubMed: 17389499) »

See all 2 Publications for this product

Product Wall

Thank you for getting back to me. I have been in touch with the source of mouse monoclonal [2Q178] to Nestin - Neural Stem Cell Marker (ab6142). My colleague in the lab considered this a possible problem. However, given that this antibody is a mous...

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Thank you for your enquiry. I have been in touch with my colleagues in the lab and I have been informed that this antibody was initially tested using NTera2 cells; a mouse neural cell line. This antibody has been shown to work by western blotting an...

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