Anti-Nestin antibody - Neural Stem Cell Marker (ab7659)

Overview

  • Product nameAnti-Nestin antibody - Neural Stem Cell Marker
    See all Nestin primary antibodies
  • Description
    Rabbit polyclonal to Nestin - Neural Stem Cell Marker
  • SpecificityDetects a band at 200kDa representing Nestin in MEFs. Denaturing of WB protein lysate causes loss of Nestin immunoreactivity in WB.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
    Predicted to work with: Rat, Rabbit, Hamster
  • Immunogen

    Synthetic peptide:

    ALETESQDSAEPSGSEE

    , corresponding to amino acids 1481-1497 of Human Nestin.

  • General notesab7659 has been upgraded from Fast Track status based on data obtained in a new batch of this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab7659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Detects a band of approximately 200 kDa (predicted molecular weight: 176 kDa). Do not denature WB protein lysate, denaturing results in loss of Nestin specific immunoreactivity (See Abreview).
  • Application notesIs unsuitable for ICC/IF or IHC-P.
  • Target

    • FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • Tissue specificityCNS stem cells.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • Post-translational
      modifications
      Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Information by UniProt
    • Database links
    • Alternative names
      • ESTM 46 antibody
      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • Nes antibody
      • Nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    Anti-Nestin antibody - Neural Stem Cell Marker images

    • Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1/2000 dilution + MEF whole cell lysate at 30 µg

      Secondary
      IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 176 kDa
      Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1 µg/ml

      Lane 1 : Mouse neural precursors whole cell lysate reduced but not denatured
      Lane 2 : Mouse neural precursors whole cell lysate reduced and denatured

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit IgG HRP at 1/20000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 176 kDa
      Observed band size : 230 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 minutes

      Randal Moldrich, CNRS UMR7637, ESPCI, France

    References for Anti-Nestin antibody - Neural Stem Cell Marker (ab7659)

    This product has been referenced in:
    • Tokumoto Y  et al. Comparison of efficiency of terminal differentiation of oligodendrocytes from induced pluripotent stem cells versus embryonic stem cells in vitro. J Biosci Bioeng 109:622-8 (2010). Read more (PubMed: 20471604) »
    • Andersen RK  et al. Effect of leukemia inhibitory factor on long-term propagation of precursor cells derived from rat forebrain subventricular zone and ventral mesencephalon. Exp Neurol 211:301-10 (2008). Read more (PubMed: 18377897) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting Abcam regarding ab7659. These applications have been listed as unsuitable as we have obtained a range of feedback stating that the antibody is staining nonspecifically in these applications.  If you have not used this a...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (Brain)
    Specification Brain
    Fixative Acetone
    Permeabilization Yes - Methanol
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 18°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 18 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (neural precursor cells/neurospheres)
    Loading amount 10 µg
    Specification neural precursor cells/neurospheres
    Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5%
    Username

    Dr. Randal Moldrich

    Verified customer

    Submitted May 18 2006

    Ab7659 is a Fast Track antibody - Fast Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen and have been released early at a reduced price. Ab7659 has not yet been tested for applica...

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    This antibody is an investigative grade antibody, which means it has not been fully characterised by Abcam but has been released due to public interest. It has only been tested in ELISA so far and its lower price reflects that. It has only been shown t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"