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Anti-Nestin antibody - Neural Stem Cell Marker (ab7659)

Overview

  • Product nameAnti-Nestin antibody - Neural Stem Cell Marker
    See all Nestin primary antibodies
  • Description
    Rabbit polyclonal to Nestin - Neural Stem Cell Marker
  • SpecificityDetects a band at 200kDa representing Nestin in MEFs. Denaturing of WB protein lysate causes loss of Nestin immunoreactivity in WB.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
    Predicted to work with: Rat, Rabbit, Hamster
  • Immunogen

    Synthetic peptide:

    ALETESQDSAEPSGSEE

    , corresponding to amino acids 1481-1497 of Human Nestin.

  • General notesab7659 has been upgraded from Fast Track status based on data obtained in a new batch of this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab7659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Detects a band of approximately 200 kDa (predicted molecular weight: 176 kDa). Do not denature WB protein lysate, denaturing results in loss of Nestin specific immunoreactivity (See Abreview).
  • Application notesIs unsuitable for ICC/IF or IHC-P.
  • Target

    • FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • Tissue specificityCNS stem cells.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • Post-translational
      modifications
      Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Information by UniProt
    • Database links
    • Alternative names
      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    Anti-Nestin antibody - Neural Stem Cell Marker images

    • Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1/2000 dilution + MEF whole cell lysate at 30 µg

      Secondary
      IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 176 kDa
      Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1 µg/ml

      Lane 1 : Mouse neural precursors whole cell lysate reduced but not denatured
      Lane 2 : Mouse neural precursors whole cell lysate reduced and denatured

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit IgG HRP at 1/20000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 176 kDa
      Observed band size : 230 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 minutes

      Randal Moldrich, CNRS UMR7637, ESPCI, France

    References for Anti-Nestin antibody - Neural Stem Cell Marker (ab7659)

    This product has been referenced in:
    • Tokumoto Y  et al. Comparison of efficiency of terminal differentiation of oligodendrocytes from induced pluripotent stem cells versus embryonic stem cells in vitro. J Biosci Bioeng 109:622-8 (2010). Read more (PubMed: 20471604) »
    • Andersen RK  et al. Effect of leukemia inhibitory factor on long-term propagation of precursor cells derived from rat forebrain subventricular zone and ventral mesencephalon. Exp Neurol 211:301-10 (2008). Read more (PubMed: 18377897) »

    See all 2 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Monkey Cell (Olfactory Stem Cells)
    Permeabilization Yes - 0,1% Triton X100
    Specification Olfactory Stem Cells
    Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative Formaldehyde
    Username

    Dr. Antoine Veron

    Verified customer

    Submitted Sep 11 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Sheep Cell (Olfactory Stem Cell)
    Permeabilization Yes - 0,1% Triton X100
    Specification Olfactory Stem Cell
    Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative Formaldehyde
    Username

    Dr. Antoine Veron

    Verified customer

    Submitted Sep 08 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Horse Cell (Olfactory Stem cells)
    Permeabilization Yes - 0,1% Triton X100
    Specification Olfactory Stem cells
    Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Dr. Antoine Veron

    Verified customer

    Submitted Jul 16 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rabbit Cell (Olfactory stem cells)
    Permeabilization Yes - 0,1% Triton 100X
    Specification Olfactory stem cells
    Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Dr. Antoine Veron

    Verified customer

    Submitted Jun 22 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Dog Cell (Olfactory Stem Cells)
    Permeabilization Yes - 0,1% Triton X100
    Specification Olfactory Stem Cells
    Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Dr. Antoine Veron

    Verified customer

    Submitted Jun 22 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Microcebus (Mouse Lemur) Cell (Olfactory Stem Cells)
    Permeabilization Yes - 0,1% Triton X100
    Specification Olfactory Stem Cells
    Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Dr. Antoine Veron

    Verified customer

    Submitted Jun 22 2015

    Thank you for contacting Abcam regarding ab7659. These applications have been listed as unsuitable as we have obtained a range of feedback stating that the antibody is staining nonspecifically in these applications.  If you have not used this a...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (Brain)
    Specification Brain
    Fixative Acetone
    Permeabilization Yes - Methanol
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 18°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 18 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (neural precursor cells/neurospheres)
    Loading amount 10 µg
    Specification neural precursor cells/neurospheres
    Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5%
    Username

    Dr. Randal Moldrich

    Verified customer

    Submitted May 18 2006

    Ab7659 is a Fast Track antibody - Fast Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen and have been released early at a reduced price. Ab7659 has not yet been tested for applica...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"