Anti-Nestin antibody - Neural Stem Cell Marker (ab92391)

Overview

  • Product nameAnti-Nestin antibody - Neural Stem Cell MarkerSee all Nestin primary antibodies ...
  • Description
    Rabbit polyclonal to Nestin - Neural Stem Cell Marker
  • SpecificityThe immunoreactivity detected with Rabbit polyclonal to Nestin (ab92391) does not overlap in with that of the mature neuronal markers such as MAP2, NeuN, Neurofilament L and Beta-3-tublin (data available on request).
  • Tested applicationsICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1600 to the C-terminus of Human Nestin.

    (Peptide available as ab19375.)

  • Positive control
    • This antibody gave a positive signal in P0 rat retinal cell culture and E16 rat hippocampal cell culture.
  • General notesab92391 is raised using the same immunogen as ab5968. Batches sold under ab92391 have passed QC testing in ICC/IF only. In our hands, this product did not work in IHC-P. For further information please contact our Customer Services team.

Properties

Applications

Our Abpromise guarantee covers the use of ab92391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
  • Application notesIs unsuitable for IHC-FoFr or IHC-P.
  • Target

    • FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • Tissue specificityCNS stem cells.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • Post-translational
      modifications
      Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Information by UniProt
    • Database links
    • Alternative names
      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • Nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    Anti-Nestin antibody - Neural Stem Cell Marker images

    • Immunocytochemical localisation of Nestin using ab92391 on P0 rat retinal culture. The image shows a nestin positive retinal ganglionic cell from P0 retina that has been grown for 7 days on a glass coverslip. Cover slips were fixed in 5% PFA in 0.1M PBS (1xPBS) for 5 minutes and washed with 0.1M PBS. Permeabilisation: 0.3% Triton X-100. Primary antibody ab92391 was diluted 1/250 and incubated for 2h @ RT. Secondary antibody: donkey anti-rabbit Alexa Fluor 488 (1/2000).
    • Immunocytochemical localisation of Nestin using ab92391 on E16 rat hippocampal cell culture. The image shows nestin positive rat hippocampal cells isolated from embryonic day 16 (grown in serum free conditions for 2-3 weeks). Cover slips were fixed in 5% PFA in 0.1M PBS (1xPBS) for 5 minutes and washed with 0.1M PBS. Permeabilisation: 0.3% Triton X-100. Primary antibody ab92391 was diluted 1/250 and incubated for 2h @ RT. Secondary antibody: donkey anti-rabbit Alexa Fluor 488 (1/2000). DAPI in blue stains nuclei.
    • ICC/IF image of ab92391 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92391, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-Nestin antibody - Neural Stem Cell Marker (ab92391)

    This product has been referenced in:
    • Jaszberenyi M  et al. Potentiation of cytotoxic chemotherapy by growth hormone-releasing hormone agonists. Proc Natl Acad Sci U S A 111:781-6 (2014). WB . Read more (PubMed: 24379381) »
    • Yan X  et al. ADAM10 negatively regulates neuronal differentiation during spinal cord development. PLoS One 9:e84617 (2014). ICC/IF . Read more (PubMed: 24404179) »

    See all 3 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (corneal endothelium)
    Specification corneal endothelium
    Fixative Paraformaldehyde
    Permeabilization Yes - 1% triton
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
    Username

    Dr. Zhiguo He

    Verified customer

    Submitted Nov 01 2012

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    Submitted Sep 21 2012

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Brain sections)
    Specification Brain sections
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Username

    Dr. Sophie Pezet

    Verified customer

    Submitted May 26 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"