Anti-Nestin antibody [SP103] (ab105389)

Overview

• Product nameAnti-Nestin antibody [SP103]See all Nestin primary antibodies ...
• Description
  Rabbit monoclonal [SP103] to Nestin
• Tested applicationsICC/IF, Flow Cyt, WB, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide corresponding to the C terminus of Human Nestin.

• Positive controlHeLa cell lysate and Human kidney tissue. SKNSH cell line

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.6
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab105389 is purified by protein A/G Chromatography.
• Clonality Monoclonal
• Clone numberSP103
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Lineage specification
  • Ectoderm

  • Stem Cells
  • Neural Stem Cells
  • Neuron Restricted Lineage

  • Stem Cells
  • Lineage Markers
  • Ectoderm

  • Stem Cells
  • Neural Stem Cells
  • Intracellular

  • Cancer
  • Tumor biomarkers
  • Tumor antigens

  • Cancer
  • Invasion/microenvironment
  • Angiogenesis
  • Other

  • Cardiovascular
  • Angiogenesis
  • Angiogenic Factors

  • Neuroscience
  • Neurology process
  • Neurogenesis

  • Neuroscience
  • Cell Adhesion Proteins
  • Cytoskeletal Proteins
  • Intermediate Filaments

  • Neuroscience
  • Cell Type Marker
  • Neural Stem Cell marker

Applications

Target

• FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
• Tissue specificityCNS stem cells.
• Sequence similaritiesBelongs to the intermediate filament family.
• Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
• Post-translational
  modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Target information above from: UniProt accession P48681 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10763 Human
  • Omim: 600915 Human
  • SwissProt: P48681 Human
  • Unigene: 527971 Human

• Alternative names
  ESTM 46 antibodyFLJ 21841 antibodyFLJ21841 antibody

  Intermediate filament protein antibodyNbla00170 antibodynes antibodyNEST_HUMAN antibodyNestin antibody

  see all

Anti-Nestin antibody [SP103] images

• 

  Western blot - Nestin antibody [SP103] (ab105389)
  Anti-Nestin antibody [SP103] (ab105389) at 1/100 dilution + HeLa cell lysate
  
  Predicted band size : 177 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nestin antibody [SP103] (ab105389)
  ab105389, at a 1/100 dilution, staining Nestin in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [SP103] (ab105389)This image is courtesy of Hongwei Shao by Abreview.
  ab105389 staining Nestin in human melanoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  
  Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using Tris EDTA pH 9.0. Samples were then permeabilized using 0.25% Triton X-100, blocked, then incubated with ab105389 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 594 (red) conjugated donkey anti-rabbit polyclonal used at a 1/100 dilution. (Green) tumor cells, (blue) DAPI.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [SP103] (ab105389)
  ICC/IF image of ab105389 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105389, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
• 

  Flow Cytometry-Anti-Nestin antibody [SP103](ab105389)
  Overlay histogram showing SH-SY5Y cells stained with ab105389 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105389, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.