Anti-Nestin antibody (ab134017)
Key features and details
- Chicken polyclonal to Nestin
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse
- Isotype: IgY
Overview
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Product name
Anti-Nestin antibody
See all Nestin primary antibodies -
Description
Chicken polyclonal to Nestin -
Host species
Chicken -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Three synthetic peptides, conjugated to KLH, corresponding to regions within Mouse Nestin (NP_057910).
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Positive control
- e13 mouse brain
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
IgY fractions were purified from the yolks and IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 300 µg/ml, and the preparation filter-sterilized. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/10000 - 1/20000.
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IHC-P | (1) |
1/10000 - 1/20000.
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WB |
1/20000 - 1/50000. Predicted molecular weight: 177 kDa.
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Notes |
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ICC/IF
1/10000 - 1/20000. |
IHC-P
1/10000 - 1/20000. |
WB
1/20000 - 1/50000. Predicted molecular weight: 177 kDa. |
Target
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Function
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. -
Tissue specificity
CNS stem cells. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments. -
Post-translational
modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. - Information by UniProt
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Database links
- Entrez Gene: 18008 Mouse
- SwissProt: Q6P5H2 Mouse
- Unigene: 331129 Mouse
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Alternative names
- ESTM 46 antibody
- FLJ 21841 antibody
- FLJ21841 antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of 3T3 cells labelling Nestin (green) with ab134017. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-Beta-Tubulin I, Blue - Hoechst stain for nuclei.
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Immunohistochemical analysis of a tissue section through an e13 mouse brain labelling Nestin (green staining) in neural stem cells of the ventricular zone of the CNS with ab134017 at 1/10000 dilution. DAPI (blue staining) allows visualization of nuclei.
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Anti-Nestin antibody (ab134017) at 1/10000 dilution + neonatal mouse brain
Predicted band size: 177 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Nestin (red) with ab134017. Tissue was fixed with 10% formalin. Green - rabbit anti-Synaptotagmin, Blue - Hoechst stain for nuclei.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (28)
ab134017 has been referenced in 28 publications.
- Qi J et al. Circ-Ctnnb1 Regulates Neuronal Injury in Spinal Cord Injury through the Wnt/β-Catenin Signaling Pathway. Dev Neurosci 44:131-141 (2022). PubMed: 34929706
- Li T et al. BMP4 Exerts Anti-Neurogenic Effect via Inducing Id3 during Aging. Biomedicines 10:N/A (2022). PubMed: 35625884
- Nourse JL et al. Piezo1 regulates cholesterol biosynthesis to influence neural stem cell fate during brain development. J Gen Physiol 154:N/A (2022). PubMed: 36069933
- Morcom L et al. DCC regulates astroglial development essential for telencephalic morphogenesis and corpus callosum formation. Elife 10:N/A (2021). PubMed: 33871356
- Dang J et al. Glial cell diversity and methamphetamine-induced neuroinflammation in human cerebral organoids. Mol Psychiatry 26:1194-1207 (2021). PubMed: 32051547