Overview

  • Product nameAnti-Nestin antibodySee all Nestin primary antibodies ...
  • Description
    Chicken polyclonal to Nestin
  • Tested applicationsIHC-P, ICC/IF, IHC-FoFr, WB more details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Mouse brain
  • General notes


    The concentrations of each of the three antibodies used to make this product was 100 µg/ml, making the total antibody concentration 300 µg/ml.

Properties

Applications

Our Abpromise guarantee covers the use of ab81755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use at an assay dependent dilution. PubMed: 21262804
ICC/IF 1/1000.
IHC-FoFr 1/10000 - 1/30000. Fix with 2% paraformaldehyde in PBS.
WB 1/20000 - 1/50000. Predicted molecular weight: 177 kDa.

Target

  • FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • Tissue specificityCNS stem cells.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • Post-translational
    modifications
    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Target information above from: UniProt accession P48681 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • Nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

Anti-Nestin antibody images

  • A tissue section through an e 13 mouse brain showing Nestin (green staining) in neural stem cells of the ventricular zone of the CNS. DAPI (blue staining) allows visualization of nuclei. The fixative was 2% paraformaldehyde in PBS, and the tissue was emersed in this fixative for 30 minutes at room temperature.

References for Anti-Nestin antibody (ab81755)

This product has been referenced in:
  • Svoboda DS  et al. Pocket proteins pRb and p107 are required for cortical lamination independent of apoptosis. Dev Biol 384:101-13 (2013). Read more (PubMed: 24056077) »
  • Yoshioka N  et al. The astrocytic lineage marker calmodulin-regulated spectrin-associated protein 1 (Camsap1): phenotypic heterogeneity of newly born Camsap1-expressing cells in injured mouse brain. J Comp Neurol 520:1301-17 (2012). Mouse . Read more (PubMed: 22095662) »

See all 4 Publications for this product

Product Wall

Thank you very much for your interest in anti-Nestin antibodies.

To our knowledge our anti-Nestin products have not been tested in bovine. Therefore, I can offer a discount off a future purchase if you buy an anti-Nestin antibody now, test it...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Cor1 cells)
Specification Cor1 cells
Fixative Formaldehyde
Permeabilization Yes - TWEEN
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1%
Username

Mr. Carl Hobbs

Verified customer

Submitted May 25 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (adult brain and kidney)
Specification adult brain and kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Dec 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"