Anti-Nestin antibody (ab82375)

Overview

• Product nameAnti-Nestin antibodySee all Nestin primary antibodies ...
• Description
  Rabbit polyclonal to Nestin
• Tested applicationsWB, ELISA, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  A KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S1409 of human Nestin.

• Positive controlHepG2 cell line lysates. IF/ICC: SKNSH cell line

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Lineage specification
  • Ectoderm

  • Stem Cells
  • Neural Stem Cells
  • Intracellular

  • Stem Cells
  • Lineage Markers
  • Ectoderm

  • Stem Cells
  • Neural Stem Cells
  • Neuron Restricted Lineage

  • Cancer
  • Invasion/microenvironment
  • Angiogenesis
  • Other

  • Cancer
  • Tumor biomarkers
  • Tumor antigens

  • Cardiovascular
  • Angiogenesis
  • Angiogenic Factors

  • Neuroscience
  • Neurology process
  • Neurogenesis

  • Neuroscience
  • Cell Type Marker
  • Neural Stem Cell marker

  • Neuroscience
  • Cell Adhesion Proteins
  • Cytoskeletal Proteins
  • Intermediate Filaments

Applications

Target

• FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
• Tissue specificityCNS stem cells.
• Sequence similaritiesBelongs to the intermediate filament family.
• Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
• Post-translational
  modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Target information above from: UniProt accession P48681 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10763 Human
  • Omim: 600915 Human
  • SwissProt: P48681 Human
  • Unigene: 527971 Human

• Alternative names
  ESTM 46 antibodyFLJ 21841 antibodyFLJ21841 antibody

  Intermediate filament protein antibodyNbla00170 antibodyNes antibodyNEST_HUMAN antibodyNestin antibody

  see all

Anti-Nestin antibody images

• 

  Western blot - Nestin antibody (ab82375)
  Anti-Nestin antibody (ab82375) at 1/50 dilution + HepG2 cell line lysates at 35 µg
  
  Predicted band size : 177 kDa
  Observed band size : 177 kDa
  Additional bands at : 60 and 65 kDa,95 and 120 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody (ab82375)
  ICC/IF image of ab82375 stained SKNSH cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82375, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.