Overview

  • Product nameAnti-Nestin antibodySee all Nestin primary antibodies ...
  • Description
    Rabbit polyclonal to Nestin
  • Tested applicationsWB, ELISA, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S1409 of human Nestin.

  • Positive control
    • HepG2 cell line lysates. IF/ICC: SKNSH cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab82375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/50 - 1/100. Predicted molecular weight: 177 kDa.
ELISA 1/1000.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • Tissue specificityCNS stem cells.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • Post-translational
    modifications
    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Target information above from: UniProt accession P48681 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • Nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

Anti-Nestin antibody images

  • Anti-Nestin antibody (ab82375) at 1/50 dilution + HepG2 cell line lysates at 35 µg

    Predicted band size : 177 kDa
    Observed band size : 177 kDa
    Additional bands at : 60 and 65 kDa,95 and 120 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab82375 stained SKNSH cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82375, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Nestin antibody (ab82375)

ab82375 has not yet been referenced specifically in any publications.

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