The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 5 - 10 µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 177 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Tissue specificityCNS stem cells.
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
Post-translational modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
All lanes : Anti-Nestin antibody (ab85684) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
developed using the ECL technique
Predicted band size : 177 kDa
Exposure time : 3 minutes
Immunoprecipitation - Nestin antibody (ab85684)
Nestin was immunoprecipitated using ab85684 at 10 µg/mg of whole cell lysate from HeLa cells.
20% of the IP was loaded, and probed with ab85684 at 0.4 µg/ml. Detection: chemiluminescence with exposure time of 30 seconds.
References for Anti-Nestin antibody (ab85684)
has not yet been referenced specifically in any publications.
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