Overview

  • Product nameAnti-Nestin antibodySee all Nestin primary antibodies ...
  • Description
    Rabbit polyclonal to Nestin
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 325 and 375 of human Nestin (NP_006608.1).

  • Positive control
    • Whole cell lysate from HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab85684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
  • Application notesIP: Use at 5 - 10 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 177 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • Tissue specificityCNS stem cells.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • Post-translational
      modifications
      Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Target information above from: UniProt accession P48681 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • Nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    Anti-Nestin antibody images

    • All lanes : Anti-Nestin antibody (ab85684) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      developed using the ECL technique

      Predicted band size : 177 kDa


      Exposure time : 3 minutes
    • Nestin was immunoprecipitated using ab85684 at 10 µg/mg of whole cell lysate from HeLa cells.
      20% of the IP was loaded, and probed with ab85684 at 0.4 µg/ml.
      Detection: chemiluminescence with exposure time of 30 seconds.

    References for Anti-Nestin antibody (ab85684)

    ab85684 has not yet been referenced specifically in any publications.

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