Anti-Nestin antibody (ab93666)

Overview

  • Product nameAnti-Nestin antibody
    See all Nestin primary antibodies
  • Description
    Rabbit polyclonal to Nestin
  • Tested applicationsICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide derived from an internal region of Human Nestin protein.

  • EpitopeInternal region
  • Positive control
    • Human liver carcinoma tissue IF/ICC: SKNSH cells

Properties

Applications

Our Abpromise guarantee covers the use of ab93666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
Flow Cyt 1/250.
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. 1/200 for 30 minutes at room temperature. Boil tissue section in 1mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Target

  • FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • Tissue specificityCNS stem cells.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • Post-translational
    modifications
    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Information by UniProt
  • Database links
  • Alternative names
    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • Nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

Anti-Nestin antibody images

  • ab93666, at a 1/200 dilution, staining Nestin in formalin fixed, paraffin embedded Human liver carcinoma tissue by Immunohistochemistry.
  • ICC/IF image of ab93666 stained SKNSH cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93666, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Nestin antibody (ab93666)

ab93666 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (neural stem cell)
Specification neural stem cell
Fixation BD cytofix-cytoperm
Permeabilization Yes - BD cytofix-cytoperm
Gating Strategy Doublets were excluded
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Verified customer

Submitted Jan 16 2012

DISCOUNT CODE: ******** Expiration date: ******** I am very pleased to hear you would like to accept our offer and test ab93666 in FACS. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, p...

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