Anti-Nestin antibody (ab93666)

Overview

  • Product nameAnti-Nestin antibodySee all Nestin primary antibodies ...
  • Description
    Rabbit polyclonal to Nestin
  • Tested applicationsICC/IF, Flow Cyt, IHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide derived from an internal region of Human Nestin protein.

  • EpitopeInternal region
  • Positive control
    • Human liver carcinoma tissue IF/ICC: SKNSH cells

Properties

Applications

Our Abpromise guarantee covers the use of ab93666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF 1/200.
Flow Cyt 1/250.
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. 1/200 for 30 minutes at room temperature. Boil tissue section in 1mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Target

  • FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • Tissue specificityCNS stem cells.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • Post-translational
    modifications
    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Target information above from: UniProt accession P48681 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • Nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

Anti-Nestin antibody images

  • ab93666, at a 1/200 dilution, staining Nestin in formalin fixed, paraffin embedded Human liver carcinoma tissue by Immunohistochemistry.
  • ICC/IF image of ab93666 stained SKNSH cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93666, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Nestin antibody (ab93666)

ab93666 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Sample Human Cell (neural stem cell)
Specification neural stem cell
Fixation BD cytofix-cytoperm
Permeabilization Yes - BD cytofix-cytoperm
Gating Strategy Doublets were excluded
Username

Abcam user community

Verified customer

Submitted Jan 16 2012

DISCOUNT CODE: ******** Expiration date: ******** I am very pleased to hear you would like to accept our offer and test ab93666 in FACS. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, p...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"