Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

Overview

  • Product nameAnti-NeuN antibody [EPR12763] - BSA and Azide free
    See all NeuN primary antibodies
  • Description
    Rabbit monoclonal [EPR12763] to NeuN - BSA and Azide free
  • Tested applicationsSuitable for: IHC-FoFr, Flow Cyt, IHC-Fr, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Goat, Cat, Dog, Human, Zebrafish, Common marmoset
  • Immunogen

    Synthetic peptide within Human NeuN aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: A6NFN3

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Cerebellum; Rat cerebellum; Human Fetal brain as well as the following whole cell lysates: 293T, HeLa and SH-SY5Y lysates; SH-SY-5Y cells. Flow Cyt: U-87MG cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-NeuN antibody (Alexa Fluor® 488) [EPR12763] - Neuronal Marker (ab190195)

    Anti-NeuN antibody (Alexa Fluor® 647) [EPR12763] - Neuronal Marker (ab190565)

Properties

Applications

Our Abpromise guarantee covers the use of ab209898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

WB Use at an assay dependent concentration. Detects a band of approximately 48.50 kDa (predicted molecular weight: 34 kDa).
ICC/IF Use at an assay dependent concentration.

Target

References for Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

This product has been referenced in:
  • Borger E  et al. The calcium-binding protein EFhd2 modulates synapse formation in vitro and is linked to human dementia. J Neuropathol Exp Neurol 73:1166-82 (2014). IHC . Read more (PubMed: 25383639) »
  • Gondo A  et al. Sustained Down-regulation of ß-Dystroglycan and Associated Dysfunctions of Astrocytic Endfeet in Epileptic Cerebral Cortex. J Biol Chem 289:30279-88 (2014). IHC-P ; Mouse . Read more (PubMed: 25228692) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"