Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOX3/NeuN [EPR12763] antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ctric acid
Sample
Cat Tissue sections (Cerebellum)
Specification
Cerebellum
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250

Additional data

Additional Notes
Submitted image shows clear, strong immunostaining of neurones in the Granule cell layer of the cerebellum.
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 29 2013

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