Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-NeuN [EPR12763] antibody - Neuronal Marker

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6
Sample
Mouse Tissue sections (Embryonic day 15 brain)
Specification
Embryonic day 15 brain
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS with 1% serum, 0.1% Triton X-100
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor­ 594 Donkey Anti-Rabbit IgG (H+L) Anti
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/700
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Submitted May 16 2014

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