Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NeuN [EPR12763] antibody - Neuronal Marker

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 4°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6.0
Sample Mouse Tissue sections (Mouse brain)
Specification Mouse brain
Permeabilization No
Fixative Paraformaldehyde

Other product details

Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% FBS in 0.1M Tris buffer pH7.6
Dilution 1/1000

Secondary antibody

Name Non-Abcam antibody was used: Molecular Probes A11037 goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution 1/1000

Additional data

Additional Notes Image shows NeuN in red, and nuclei (DAPI) in blue. Staining was primarily nuclear with some minor cytoplasmic fluorescence.

Dr. Adam Walker

Verified customer

Submitted Jun 19 2014