Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NeuN [EPR12763] antibody - Neuronal Marker

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 4°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6.0
Sample
Mouse Tissue sections (Mouse brain)
Specification
Mouse brain
Permeabilization
No
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% FBS in 0.1M Tris buffer pH7.6
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Molecular Probes A11037 goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000

Additional data

Additional Notes
Image shows NeuN in red, and nuclei (DAPI) in blue. Staining was primarily nuclear with some minor cytoplasmic fluorescence.
Username

Dr. Adam Walker

Verified customer

Submitted Jun 19 2014

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