Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-NeuN antibody [EPR12763] - Neuronal Marker

Excellent
Abreviews
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Coronal Brain Sections)
Antigen retrieval step None
Permeabilization Yes - 0.3% Triton X in PBS
Specification Coronal Brain Sections
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde

Other product details

Incubation time 24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: incubated overnight
Dilution 1/500

Secondary antibody

Dilution 1/500

Additional data

Additional Notes Image is 40x magnification of the dentate gyrus granule neurons located in a 20um coronal section of rat hippocampus.
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Mr. Kyle Klingbeil

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Submitted Aug 12 2015