Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-NeuN antibody [EPR12763] - Neuronal Marker

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Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (brain)
Antigen retrieval step None
Permeabilization Yes - 0.1% saponin
Specification brain
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde

Other product details

Incubation time 18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS/5% goat serum/0.1% saponin
Dilution 1/500

Secondary antibody

Dilution 1/1000
Name Non-Abcam antibody was used: goat anti-rabbit alexa 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 594

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Submitted Feb 23 2017