Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-NeuN antibody [EPR12763] - Neuronal Marker

Excellent
Abreviews
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Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% saponin
Specification
brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS/5% goat serum/0.1% saponin
Dilution
1/500

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: goat anti-rabbit alexa 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 594
Username

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Submitted Feb 23 2017

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