Anti-Neurexin 1 antibody (ab77596)
Key features and details
- Goat polyclonal to Neurexin 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Neurexin 1 antibody
See all Neurexin 1 primary antibodies -
Description
Goat polyclonal to Neurexin 1 -
Host species
Goat -
Specificity
This antibody will detect Neurexin 1 beta and Neurexin 1 alpha (isoform 1a, 2a and 3a). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog -
Immunogen
Synthetic peptide corresponding to Human Neurexin I beta (C terminal). The immunogen sequence is aa419-432 of Neurexin 1 beta (P58400), aa1454-1467 of Neurexin 1 alpha (Q9ULB1), aa1476-1489 of Neurexin 1 alpha (Q9ULB1-2), and aa1524-1537 of Neurexin 1 alpha (Q9ULB1-3).
Sequence:KEKQPSSAKSSNKN
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.05 - 0.1 µg/ml. Predicted molecular weight: 162 kDa.
A 1 hour primary incubation is recommended for this product. |
Notes |
---|
WB
Use a concentration of 0.05 - 0.1 µg/ml. Predicted molecular weight: 162 kDa. A 1 hour primary incubation is recommended for this product. |
Target
-
Function
Cell surface protein involved in cell-cell-interactions, exocytosis of secretory granules and regulation of signal transmission. Function is isoform-specific. Alpha-type isoforms have a long N-terminus with six laminin G-like domains and play an important role in synaptic signal transmission. Alpha-type isoforms play a role in the regulation of calcium channel activity and Ca(2+)-triggered neurotransmitter release at synapses and at neuromuscular junctions. They play an important role in Ca(2+)-triggered exocytosis of secretory granules in pituitary gland. They may effect their functions at synapses and in endocrine cells via their interactions with proteins from the exocytotic machinery. Likewise, alpha-type isoforms play a role in regulating the activity of postsynaptic NMDA receptors, a subtype of glutamate-gated ion channels. Both alpha-type and beta-type isoforms may play a role in the formation or maintenance of synaptic junctions via their calcium-dependent interactions (via the extracellular domains) with neuroligin family members, CBLN1 or CBLN2. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Alpha-type isoforms were first identified as receptors for alpha-latrotoxin from spider venom. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the neurexin family.
Contains 3 EGF-like domains.
Contains 6 laminin G-like domains. -
Post-translational
modificationsN-glycosylated.
O-glycosylated. -
Cellular localization
Cell membrane. Cell junction, synapse. Localized on the pre-synaptic membrane. - Information by UniProt
-
Database links
- Entrez Gene: 9378 Human
- Entrez Gene: 18189 Mouse
- Entrez Gene: 60391 Rat
- Omim: 600565 Human
- SwissProt: P58400 Human
- SwissProt: Q9ULB1 Human
- SwissProt: Q9CS84 Mouse
- SwissProt: Q63372 Rat
see all -
Form
The neurexin genes use alternate promoters, splice sites and exons to encode potentially hundreds or thousands of mRNA transcripts. -
Alternative names
- DKFZp313P2036 antibody
- FLJ35941 antibody
- Hs.22998 antibody
see all
Images
-
Anti-Neurexin 1 antibody (ab77596) at 0.05 µg/ml + human brain (cerebellum) lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 162 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Primary antibody incubation: 1 hour
Datasheets and documents
-
Datasheet download
References (3)
ab77596 has been referenced in 3 publications.
- Dore K et al. SYNPLA, a method to identify synapses displaying plasticity after learning. Proc Natl Acad Sci U S A 117:3214-3219 (2020). PubMed: 31974314
- Torres-Vega E et al. Netrin-1 receptor antibodies in thymoma-associated neuromyotonia with myasthenia gravis. Neurology 88:1235-1242 (2017). PubMed: 28251919
- Wei H et al. Redox/methylation mediated abnormal DNA methylation as regulators of ambient fine particulate matter-induced neurodevelopment related impairment in human neuronal cells. Sci Rep 6:33402 (2016). WB . PubMed: 27624276