Anti-NeuroD2 antibody (ab104430)
Key features and details
- Rabbit polyclonal to NeuroD2
- Suitable for: IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-NeuroD2 antibody
See all NeuroD2 primary antibodies -
Description
Rabbit polyclonal to NeuroD2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Gorilla, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Mouse NeuroD2 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab124135) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (4) |
Use at an assay dependent concentration.
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IHC-Fr | (1) |
1/2000. Tissue was perfusion fixed in 10% formaldehyde. Staining appeared more intense without antigen retrieval.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. |
IHC-Fr
1/2000. Tissue was perfusion fixed in 10% formaldehyde. Staining appeared more intense without antigen retrieval. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa). |
Target
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Function
Appears to mediate neuronal differentiation. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4761 Human
- Entrez Gene: 18013 Mouse
- Entrez Gene: 54276 Rat
- Omim: 601725 Human
- SwissProt: Q15784 Human
- SwissProt: Q62414 Mouse
- SwissProt: Q63689 Rat
- Unigene: 322431 Human
see all -
Alternative names
- bHLHa1 antibody
- class A basic helix loop helix protein 1 antibody
- Class A basic helix-loop-helix protein 1 antibody
see all
Images
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IHC image of NeuroD2 staining in a formalin fixed, paraffin embedded mouse brain tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104430, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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All lanes : Anti-NeuroD2 antibody (ab104430) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Cerebellum Mouse Tissue Lysate
Lane 3 : Mouse Hippocampus Tissue Lysate
Lane 4 : P0 Mouse Brain Mouse Tissue Lysate
Lane 5 : P7 Mouse Brain Tissue Lysate
Lane 6 : Human brain tissue lysate - total protein (ab29466) with Immunising peptide at 1 µg/ml
Lane 7 : Cerebellum Mouse Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 8 : Mouse Hippocampus Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 9 : P0 Mouse Brain Mouse Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 10 : P7 Mouse Brain Tissue Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 21 kDa, 41 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
IHC-P image of NeuroD2 staining on mouse CA1 hippocampus sections using ab104430 (1:2000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections wer then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours.
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IHC-P image of NeuroD2 staining on rat CA1 hippocampus sections using ab104430 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections wer then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours
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ab104430 staining NeuroD2 in 10% formaldehyde perfusion fixed 6 week old frozen mouse brain section (dentate gyrus). No antigen retrieval was performed. The section was incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104430, 1/2000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab104430 has been referenced in 22 publications.
- Tomasello U et al. miR-137 and miR-122, two outer subventricular zone non-coding RNAs, regulate basal progenitor expansion and neuronal differentiation. Cell Rep 38:110381 (2022). PubMed: 35172154
- Zhu X et al. BRN2 as a key gene drives the early primate telencephalon development. Sci Adv 8:eabl7263 (2022). PubMed: 35245119
- Wang J et al. A Ctnnb1 enhancer regulates neocortical neurogenesis by controlling the abundance of intermediate progenitors. Cell Discov 8:74 (2022). PubMed: 35915089
- Liu JW et al. Npas3 regulates stemness maintenance of radial glial cells and neuronal migration in the developing mouse cerebral cortex. Front Cell Neurosci 16:865681 (2022). PubMed: 36313621
- Yi J et al. Histone demethylase UTX/KDM6A enhances tumor immune cell recruitment, promotes differentiation and suppresses medulloblastoma. Cancer Lett 499:188-200 (2021). PubMed: 33253789