Anti-Neurofilament antibody [RNF406] (ab134306)
Key features and details
- Mouse monoclonal [RNF406] to Neurofilament
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-Neurofilament antibody [RNF406]
See all Neurofilament primary antibodies -
Description
Mouse monoclonal [RNF406] to Neurofilament -
Host species
Mouse -
Specificity
ab134306 reacts exclusively with the phosphorylated isoform of the 160 kDa neurofilament protein. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Guinea pig, Hamster, Cow, Xenopus laevis, Monkey -
Immunogen
Neurofilament preparation of calf brain tissue.
-
Positive control
- Human brain cortex tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
Clonality
Monoclonal -
Clone number
RNF406 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/100 - 1/500. Predicted molecular weight: 102 kDa.
|
|
IHC-P |
Use a concentration of 10 µg/ml.
|
Notes |
---|
WB
1/100 - 1/500. Predicted molecular weight: 102 kDa. |
IHC-P
Use a concentration of 10 µg/ml. |
Target
-
Function
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsThere are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
-
Database links
- Entrez Gene: 4741 Human
- Entrez Gene: 4744 Human
- Entrez Gene: 4747 Human
- Omim: 162230 Human
- Omim: 162250 Human
- Omim: 162280 Human
- SwissProt: P07196 Human
- SwissProt: P07197 Human
see all -
Alternative names
- 150kDa medium antibody
- 160 kDa neurofilament protein antibody
- 2 kDa neurofilament protein antibody
see all
Images
-
All lanes : Anti-Neurofilament antibody [RNF406] (ab134306) at 1/100 dilution
Lane 1 : Wild-type HEK293 whole cell lysate
Lane 2 : Neurofilament knockout HEK293 whole cell lysate
Lane 3 : A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 102 kDa
Observed band size: 140-160 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab134306 observed at 140-160 kDa kDa. Red - loading control, ab52866, observed at 50 kDa.
ab134306 was shown to specifically react with Neurofilament in wild-type HEK293 cells as signal was lost in Neurofilament knockout cells. Wild-type and Neurofilament knockout samples were subjected to SDS-PAGE. ab134306 and ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/100 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labelling 140 kD + 160 kD Neurofilament with ab134306 at 10 µg/ml, using a biotinylated horse anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Protocols
Datasheets and documents
-
Datasheet download
References (6)
ab134306 has been referenced in 6 publications.
- Fan H et al. Exosomes derived from olfactory ensheathing cells provided neuroprotection for spinal cord injury by switching the phenotype of macrophages/microglia. Bioeng Transl Med 7:e10287 (2022). PubMed: 35600663
- Wang N et al. MicroRNA-135a-5p Promotes the Functional Recovery of Spinal Cord Injury by Targeting SP1 and ROCK. Mol Ther Nucleic Acids 22:1063-1077 (2020). PubMed: 33294293
- Zhang J et al. Ulinastatin Promotes Regeneration of Peripheral Nerves After Sciatic Nerve Injury by Targeting let-7 microRNAs and Enhancing NGF Expression. Drug Des Devel Ther 14:2695-2705 (2020). PubMed: 32753848
- Wu R et al. Decreased miR-325-5p Contributes to Visceral Hypersensitivity Through Post-transcriptional Upregulation of CCL2 in Rat Dorsal Root Ganglia. Neurosci Bull N/A:N/A (2019). PubMed: 30980241
- Rolland-Fourcade C et al. Epithelial expression and function of trypsin-3 in irritable bowel syndrome. Gut 66:1767-1778 (2017). PubMed: 28096305
- Bryukhovetskiy IS et al. Hematopoietic stem cells as a tool for the treatment of glioblastoma multiforme. Mol Med Rep 14:4511-4520 (2016). Human . PubMed: 27748891