For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to Neurogenin 1 - N-terminal
Synthetic peptide from the N-terminal region of human Neurogenin 1 conjugated to KLH
A375 cell lysate. Human hepatocarcinoma tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
ab71818 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/100 - 1/500. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa).
Appears to mediate neuronal differentiation.
Expression restricted to the embryonic nervous system.
Contains 1 basic helix-loop-helix (bHLH) domain.
Information by UniProt
Western blot - Neurogenin 1 antibody - N-terminal (ab71818)
Anti-Neurogenin 1 antibody - N-terminal (ab71818) at 1/100 dilution + A375 cell lysate at 12.5 µg
Predicted band size:
Observed band size:
23 kDa (
why is the actual band size different from the predicted?
Additional bands at:
33 kDa, 45 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neurogenin 1 antibody - N-terminal (ab71818)
ab71818 at 1/50 dilution, staining Neurogenin 1 in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"