Overview
- Product nameAnti-Neurogenin3 antibody [3B5]See all Neurogenin3 primary antibodies ...
- DescriptionMouse monoclonal [3B5] to Neurogenin3
- Tested applicationsWB, IHC-P, ICC/IF more details
- Species reactivityReacts with: Human
- Immunogen
Protein expressed in 293T cells transfected with Human Neurogenin3 expression vector.
- Positive controlTransiently transfected HEK293T cell lysate. Human liver tissue. Transiently transfected HeLa cells.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.3 -
Concentration information loading... - PurityProtein A purified
- Purification notesab87108 is purified from mouse ascites fluids by affinity chromatography.
- Clonality Monoclonal
- Clone number3B5
- IsotypeIgG2a
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab87108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/2000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| ICC/IF | ICC/IF: 1/100. |
Target
- FunctionInvolved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types.
- Involvement in diseaseDefects in NEUROG3 are the cause of diarrhea type 4 (DIAR4) [MIM:610370]. DIAR4 is a characterized by severe, life-threatening watery diarrhea associated with generalized malabsorption and a paucity of enteroendocrine cells.
- Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
- Cellular localizationNucleus.
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Database links
- Entrez Gene: 50674 Human
- Omim: 604882 Human
- SwissProt: Q9Y4Z2 Human
- Unigene: 532682 Human
Target information above from: UniProt accession
Q9Y4Z2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ATH4B antibodyAtoh5 antibodybHLHa7 antibody
- Class A basic helix-loop-helix protein 7 antibodyMath4B antibodyNEUROG3 antibodyNeurogenin-3 antibodyNGN-3 antibodyngn3 antibodyNGN3_HUMAN antibodyOTTHUMP00000019730 antibodyProtein atonal homolog 5 antibody
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Anti-Neurogenin3 antibody [3B5] images
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![Western blot - Neurogenin3 antibody [3B5] (ab87108)](http://a.abcam.com/ps/datasheet/images/87/ab87108/Neurogenin3-Primary-antibodies-ab87108-1.jpg)
Western blot - Neurogenin3 antibody [3B5] (ab87108)All lanes : Anti-Neurogenin3 antibody [3B5] (ab87108) at 1/2000 dilution
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY Neurogenin3 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size : 23 kDa
Observed band size : 23 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Neurogenin3 antibody [3B5] (ab87108)](http://a.abcam.com/ps/datasheet/images/87/ab87108/Neurogenin3-Primary-antibodies-ab87108-5.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Neurogenin3 antibody [3B5] (ab87108)ab87108 immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY NEUROG3. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogenin3 antibody [3B5] (ab87108)](http://a.abcam.com/ps/datasheet/images/87/ab87108/Neurogenin3-Primary-antibodies-ab87108-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogenin3 antibody [3B5] (ab87108)Immunohistochemical staining of paraffin-embedded Human lymph node tissue using ab87108.
References for Anti-Neurogenin3 antibody [3B5] (ab87108)
ab87108 has not yet been referenced specifically in any publications.
