Anti-Neurogranin antibody (ab99269)
Key features and details
- Goat polyclonal to Neurogranin
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Neurogranin antibody
See all Neurogranin primary antibodies -
Description
Goat polyclonal to Neurogranin -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog -
Immunogen
Synthetic peptide:
C-KIKSGERGRKG
, corresponding to internal sequence amino acids 45-55 of Human Neurogranin (NP_006167.1). -
Positive control
- Human brain cerebral cortex lysate, Human brain hippocampus lysate, Mouse brain lysate and Rat brain lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab99269 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 8 kDa).
A 1 hour primary incubation is recommended for this product. An approximately 15 kDa band is observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates. There is also an additional 16kDa band. The observed molecular weights correspond to earlier findings in the literature with different antibodies. |
Notes |
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IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 8 kDa). A 1 hour primary incubation is recommended for this product. An approximately 15 kDa band is observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates. There is also an additional 16kDa band. The observed molecular weights correspond to earlier findings in the literature with different antibodies. |
Target
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Function
Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium. -
Tissue specificity
In the cerebral cortex, found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. Is not found in the dendrites in patients with Alzheimer disease. -
Sequence similarities
Belongs to the neurogranin family.
Contains 1 collagen-like domain.
Contains 1 IQ domain. - Information by UniProt
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Database links
- Entrez Gene: 4900 Human
- Entrez Gene: 64011 Mouse
- Entrez Gene: 64356 Rat
- Omim: 602350 Human
- SwissProt: P35722 Cow
- SwissProt: Q92686 Human
- SwissProt: P60761 Mouse
- SwissProt: Q04940 Rat
see all -
Alternative names
- Protein kinase C substrate RC3 antibody
- Calmodulin binding protein antibody
- Hng antibody
see all
Images
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All lanes : Anti-Neurogranin antibody (ab99269) at 2 µg/ml
Lane 1 : Human cerebellum lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 35 µg per lane.
Predicted band size: 8 kDa -
Anti-Neurogranin antibody (ab99269) at 0.1 µg/ml + Mouse brain lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 8 kDa
Primary incubation was 1 hour. -
ab99269 (3.8µg/ml) staining of paraffin embedded Human Cortex shows cytoplasm staining in the neuronal cell bodies. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab99269 has been referenced in 1 publication.
- Sun X et al. Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy. Brain Behav Immun 82:178-187 (2019). PubMed: 31437533