Immunocytochemistry abreview for Anti-Neuroligin 1 antibody

Poor
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cultured Cells (neurons)
Specification
neurons
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 0.3% · Temperature: 20°C

Other product details

Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 30°C · Diluent: PBS-BSA

Secondary antibody

Name
Non-Abcam antibody was used: Chicken anti-goat
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
Several conditions were tested (time and concentration), with poor results. The affinity seems to be very low. I've also tried PFA fixatin and blocking solution of fish gelatin, and a secondary donkey anti-goat, with the same results. I attach a figure with representative images of cells incubated ON with the primary antibody. They are representative of the other incubation times tested. Signal is in the background range. As a control, I've performed succesfull immunostainings on the cells of the same batch and with the same solutions. If you find the good conditions to make it work, please tell me!
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
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Submitted Feb 07 2011

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