Several conditions were tested (time and concentration), with poor results. The affinity seems to be very low. I've also tried PFA fixatin and blocking solution of fish gelatin, and a secondary donkey anti-goat, with the same results. I attach a figure with representative images of cells incubated ON with the primary antibody. They are representative of the other incubation times tested. Signal is in the background range. As a control, I've performed succesfull immunostainings on the cells of the same batch and with the same solutions. If you find the good conditions to make it work, please tell me!
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
Abcam user community
Submitted Feb 07 2011
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