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Anti-Neuroligin 3 antibody (ab34122)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab34122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 91 kDa).Can be blocked with Neuroligin 3 peptide (ab34121).

Target

  • Alternative names
      Gliotactin homolog antibodyNlgn3 antibody

Anti-Neuroligin 3 antibody images

  • All lanes : Anti-Neuroligin 3 antibody (ab34122) at 1 µg/ml

    Lane 1 : Neuroligin-3 positive control (Wild-Type) Lysate
    Lane 2 : Neuroligin-3 negative control (Knockout) Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 91 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

References for Anti-Neuroligin 3 antibody (ab34122)

ab34122 has not yet been referenced specifically in any publications.

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Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Verified customer

Submitted Sep 19 2008

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Verified customer

Submitted Jul 25 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"