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Product name
Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker
See all Neuron specific beta III Tubulin products (5) ...
Description
Rabbit polyclonal to Neuron specific beta III Tubulin - Neuronal Marker
Specificity
The immunising sequence is found in both beta III and beta IV tubulin. ab18207 detects human neuron specific beta III Tubulin protein specifically and cleanly but not as strongly as it detects the equivalent mouse and rat protein.
Tested applications
IHC-FoFr, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Marmoset (common), Dogfish/Catshark
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human neuron specific beta III Tubulin.
(Peptide available as ab186 60.)
Positive control
Human, Mouse, Rat or Dogfish/Catshark Brain Tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Neuronal
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Microtubules
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - neuron specific beta III Tubulin antibody - Neuronal Marker (ab18207)
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Western blot - Neuron specific beta III Tubulin antibody - Neuronal Marker (ab18207)
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Immunocytochemistry/ Immunofluorescence - neuron specific beta III Tubulin antibody - Neuronal Marker (ab18207)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab18207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution. PubMed: 20568963
Flow Cyt: Use at an assay dependent concentration.
IHC-P: 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution.
ICC/IF: Use a concentration of 1 µg/ml.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 50 kDa).Can be blocked with neuron specific beta III Tubulin peptide (ab18660).
Target
Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Tissue specificity
Expression is primarily restricted to central and peripheral nervous system.
Involvement in disease
Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
Sequence similarities
Belongs to the tubulin family.
Domain
The highly acidic C-terminal region may bind cations such as calcium.
Post-translational
modifications
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionQ13509
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- beta 4 antibody
- MC1R antibody
- TBB3_HUMAN antibody
see all
Database links
- Entrez Gene: 10381 Human
- Entrez Gene: 22152 Mouse
- Entrez Gene: 246118 Rat
- Omim: 602661 Human
- SwissProt: Q13509 Human
- SwissProt: Q9ERD7 Mouse
- SwissProt: Q4QRB4 Rat
- Unigene: 511743 Human
see all
Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - neuron specific beta III Tubulin antibody - Neuronal Marker (ab18207)
Immunohistochemistical staining (Formaldehyde/PFA-fixed paraffin-embedded sections) for Neuron specific beta III Tubulin antibody - Neuronal Marker (ab18207) on Dogfish/Catshark Tissue sections (head: snout region). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody used at 1/2000 incubated for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).
Carl Hobbs, CARD, KCL, London, UK
Western blot - Neuron specific beta III Tubulin antibody - Neuronal Marker (ab18207)
All lanes : Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab18207) at 1 µg/ml
Lane 1 :
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 :
Lane 5 : Brain (Mouse) Tissue Lysate with
Lane 6 : Brain (Rat) Tissue Lysate with
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
Immunocytochemistry/ Immunofluorescence - neuron specific beta III Tubulin antibody - Neuronal Marker (ab18207)
ICC/IF image of ab18207 stained SH-SY-5Y cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18207, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - neuron specific beta III Tubulin antibody - Neuronal Marker (ab18207)
ab18207 at 1/2000 staining rat cerebellum tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 16 hours. A biotinylated goat polyclonal antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Mr Carl hobbs
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - neuron specific beta III Tubulin antibody - Neuronal Marker (ab18207)
ab18207 at 1/2000 staining mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and an enzymatic antigen retrieval step was performed prior to incubation with the antibody for 16 hours. A biotinylated goat anti-rabbit IgG was used as the secondary.
This image is courtesy of an Abreview submitted by Mr Carl hobbs
Immunohistochemistry (Frozen sections) - neuron specific beta III Tubulin antibody - Neuronal Marker (ab18207)
ab18207 staining rat somatosensory cortex tissue sections by IHC-Fr. Sections were PFA fixed and permeabilized in TritonX100 prior to blocking in 3% BSA for 1 hour at RT. The primary antibody was diluted 1/1000 and incubated with the sample for 18 hrs at 4°C. A biotinylated goat anti-rabbit IgG was used as the secondary antibody. The anti-beta III tubulin staining is clearly visible in dendrites of pyramidal cells passing from layer V to the superficial layers. (Magnification = 200x)
This image is courtesy of an Abreview submitted by Dr Grazyna Niewiadomska
Immunocytochemistry/ Immunofluorescence - neuron specific beta III Tubulin antibody - Neuronal Marker (ab18207)
ab18207 staining human cultured stem cells differentiated to neurons, by ICC/IF. Cells were PFA fixed and permeabilized in Triton X prior to blocking with 1% BSA for 30 minutes at 27°C. The primary antibody was used undiluted and incubated with the sample for 24 hours at 4°C. A PE-conjugated rabbit anti-rabbit antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
References for Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab18207)
This product has been referenced in:
- Robinson AP et al. Human stem/progenitor cells from bone marrow enhance glial differentiation of rat neural stem cells: a role for transforming growth factor ß and Notch signaling. Stem Cells Dev 20:289-300 (2011). WB, ICC/IF; Human.Read more (PubMed: 20575640) »
- Ferruzzi P et al. In vitro and in vivo characterization of a novel hedgehog signaling antagonist in human glioblastoma cell lines. Int J Cancer : (2011).Read more (PubMed: 22072503) »
See all 11 publications for this product
Publishing research using ab18207? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"