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Product name
Anti-Neuronatin antibody
See all Neuronatin products (2) ...
Description
Rabbit polyclonal to Neuronatin
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Mouse Neuronatin.
(Peptide available as ab303 32.)
Positive control
Mouse Brain Whole Tissue Lysate (0-days old). SKNSH cell line (IF).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. PubMed: 20872847
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa).
IHC-P: 1/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Target
Function
May participate in the maintenance of segment identity in the hindbrain and pituitary development, and maturation or maintenance of the overall structure of the nervous system. May function as a regulatory subunit of ion channels.
Sequence similarities
Belongs to the neuronatin family.
Developmental stage
Abundant in 18-24 week old fetal brain. Postnatally its expression decline and only minimal levels were present in adulthood.
Target information above from: UniProt accessionQ16517
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Neuronatin antibody
- Nnat antibody
- NNAT_HUMAN antibody
see all
Database links
- Entrez Gene: 4826 Human
- Entrez Gene: 18111 Mouse
- Entrez Gene: 94270 Rat
- Omim: 603106 Human
- SwissProt: Q16517 Human
- SwissProt: Q61979 Mouse
- SwissProt: Q62649 Rat
- Unigene: 504703 Human
see all
Anti-Neuronatin antibody images:
Western blot - Neuronatin antibody (ab27266)
Anti-Neuronatin antibody (ab27266) at 1 µg/ml +
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 9 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neuronatin antibody (ab27266)
ab27266 at 1/1000 staining human pituitary cells by IHC-P The antibody was tested on normal human pituitary slide sections (formalin fixed/paraffin embedded). This gave good staining over a range of concentrations with a microwave/pressure antigen retrieval step. Staining was strongly associated with the cell membrane and blocked by incubating with the neuronatin antibody with the peptide which the antibody was raised against.
This image is courtesy of an anonymous Abreview
Western blot - Neuronatin antibody (ab27266)
Anti-Neuronatin antibody (ab27266) at 1 µg/ml +
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 9 kDa
Observed band size : 10 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70-75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
Immunocytochemistry/ Immunofluorescence - Anti-Neuronatin antibody (ab27266)
ICC/IF image of ab27266 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Neuronatin antibody (ab27266)
This product has been referenced in:
- Dugu L et al. LETTER TO THE EDITOR: Abundant expression of neuronatin in normal eccrine, apocrine and sebaceous glands and their neoplasms. J Dermatol 37:846-8 (2010). IHC-P; Human.Read more (PubMed: 20883377) »
- Lin HH et al. Neuronatin Promotes Neural Lineage in ESCs via Ca(2+) Signaling. Stem Cells 28:1950-1960 (2010). WB, ICC/IF; Human.Read more (PubMed: 20872847) »
See all 4 publications for this product
Publishing research using ab27266? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"