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Immunohistochemistry (PFA fixed) - Neuropeptide S antibody (ab18252)
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Radioimmunoassay - Neuropeptide S antibody (ab18252)
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Product Name
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Neuropeptide S antibody
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See all Neuropeptide S antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Neuropeptide S
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Neuropeptide S and a synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Rat Neuropeptide S. (Note: the amino acid sequences are proprietary)
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Reacts with
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Ms, Rat
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Predicted to react
(species key)
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Hu, Cow
(Due to sequence homology)
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Specificity
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ab18252 Neuropeptide S antibody is designed to detect mature rat, mouse and human NPS peptide. ab18252 detects mouse NPS peptide in IHC (PFA fixed) and in RIA in hypothalamic mouse brain tissue (data not shown).
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Tested applications
(see key)
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IHC (PFA fixed), IHC-FoFr, IHC-Fr, RIA
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-Fo: Use at an assay dependent dilution.
Immunohistochemistry (PFA fixed): Use at a concentration of 0.3 µg/ml.
RIA: Use at a concentration of 0.1 - 0.3 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse brain
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Cellular localization
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Cytoplasmic and Secreted
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Research areas
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Neuroscience >> Endocrine system >> hypothalamic pituitary adrenal axis
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Relevance
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Neuropeptide S (NPS) is a novel target in the neurobiology of sleep/wakefulness regulation and anxiety-like behavior. NPS potently promotes arousal and suppress all stages of sleep. NPS acts via its cognate receptor (NPSR) and induces mobilization of intracellular Ca2+ stores. NPSR mRNA is widely distributed in the brain, including the amygdala and the midline thalamic nuclei. The NPS peptide precursor is primarily expressed between the locus coeruleus (LC) and Barrington's nucleus, a part of the brain with known function in arousal, stress, and anxiety.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Neuropeptide S antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Neuropeptide S antibody images, references, products related to ab18252 and other tools.
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Neuropeptide S antibody images:
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 Immunohistochemistry (PFA fixed) - Neuropeptide S antibody (ab18252)
Immunofluorescent staining using the Neuropeptide S antibody (ab18252) in the rat hypothalamus. Picture taken using objectives X20 (A) or X40 (B). The staining is observed in thin and varicose fibers. This staining is blocked by preincubation with immunising peptides (ab18698 and ab18699). ab18252 was used at 1/1000 (0.3µg/ml) incubated overnight at room temperature. Secondary antibody used was anti-rabbit Alexa Fluor 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.
Sophie Pezet, Univ London Kings Coll, United Kingdom
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Radioimmunoassay - Neuropeptide S antibody (ab18252)
IC50: 46 fmoles; ab18252 used at a dilution of 1/3000; Tracer: [125I] Y10-hNPS, 12.000 cpm; Detection method: anti-rabbit SPA (Amersham). Radioimmunoassay was performed in equilibrium, using the SPA technology (Amersham). 50µl antirabbit YSi SPA beads (RPN 140) were mixed with 50µl antiserum (1:3000), 50µl tracer (12,000 cpm) and with or without cold NPS peptide in increasing concentrations. All reagents were resuspended in 0.1M phosphate buffer, pH 7, 0.1% BSA. Incubation was for 48h at RT. Bound radioligand was detected by scintillation proximity in a beta counter (MicroBeta, Wallac).
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Search PubMed (MEDLINE) for references to Neuropeptide S
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Neuropeptide S antibody - more information
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Customer reviews (feedback) regarding Neuropeptide S antibody |
Customer FAQs regarding Neuropeptide S antibody |
Protocols for Neuropeptide S antibody |
Price and availability of products related to Neuropeptide S antibody |
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Neuropeptide S antibody - related products:
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Neuropeptide S antibody - other tools:
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Contact Abcam with a Technical Enquiry about Neuropeptide S antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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