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Products:Neuroscience >> Endocrine system >> Hypothalamic pituitary adrenal axis
Overview
Product name
Anti-Neuropeptide S antibody
See all Neuropeptide S products (4) ...
Description
Rabbit polyclonal to Neuropeptide S
Specificity
This antibody is generated against the complete 20aa sequence of the fully processed rat NPS peptide, it should run at about 2kDa on WB. ab18252 detects mouse NPS peptide in IHC (PFA fixed) and in RIA in hypothalamic mouse brain tissue.
Tested applications
IHC (PFA fixed), RIA, IHC-FoFr, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of fully processed Rat Neuropeptide S.
(Peptide available as ab18699.)
Positive control
Rat and Mouse brain
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Endocrine system >> Hypothalamic pituitary adrenal axis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab18252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-Fo: Use at an assay dependent dilution.
Immunohistochemistry (PFA fixed): Use at a concentration of 0.3 µg/ml.
RIA: Use at a concentration of 0.1 - 0.3 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Modulates arousal and anxiety. May play an important anorexigenic role. Binds to its receptor NPSR1 with nanomolar affinity to increase intracellular calcium concentrations.
Cellular localization
Secreted.
Target information above from: UniProt accessionP0C0P6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Neuropeptide S antibody
- NPS antibody
- NPS_HUMAN antibody
Database links
- Entrez Gene: 100043254 Mouse
- SwissProt: P0C0P8 Mouse
- SwissProt: P0C0P7 Rat
- Unigene: 192092 Mouse
Anti-Neuropeptide S antibody images:
Immunohistochemistry (PFA fixed) - Neuropeptide S antibody (ab18252)
Immunofluorescent staining using the Neuropeptide S antibody (ab18252) in the rat hypothalamus. Picture taken using objectives X20 (A) or X40 (B). The staining is observed in thin and varicose fibers. This staining is blocked by preincubation with immunising peptides (ab18698 and ab18699). ab18252 was used at 1/1000 (0.3µg/ml) incubated overnight at room temperature. Secondary antibody used was anti-rabbit Alexa Fluor 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.
Sophie Pezet, Univ London Kings Coll, United Kingdom
Radioimmunoassay - Neuropeptide S antibody (ab18252)
IC50: 46 fmoles; ab18252 used at a dilution of 1/3000; Tracer: [125I] Y10-hNPS, 12.000 cpm; Detection method: anti-rabbit SPA (Amersham).
Radioimmunoassay was performed in equilibrium, using the SPA technology (Amersham). 50µl antirabbit YSi SPA beads (RPN 140) were mixed with 50µl antiserum (1:3000), 50µl tracer (12,000 cpm) and with or without cold NPS peptide in increasing concentrations. All reagents were resuspended in 0.1M phosphate buffer, pH 7, 0.1% BSA. Incubation was for 48h at RT. Bound radioligand was detected by scintillation proximity in a beta counter (MicroBeta, Wallac).
Immunohistochemistry (Frozen sections) - Neuropeptide S antibody (ab18252)
ab18252 staining Neuropeptide S in mouse brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocking with 5% serum for 1 hour at 220C was performed. The sample was incubated with primary antibody (1/1000) for 10 hours at 220C. An Alexa Fluor® 555-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/500 dilution. The images were generated from same slice and same area (KF, a part of LPB on mouse brain atlas). Scale bar is 200 µm on the left image and 20 µm on the right image. Blue colour represents DAPI staining, while red one is for Neuropeptide S.
This image is a courtesy of Xiaobin Liu
References for Anti-Neuropeptide S antibody (ab18252)
This product has been referenced in:
- Liu Xet al. Molecular fingerprint of neuropeptide S-producing neurons in the mouse brain. J Comp Neurol 519:1847-66 (2011). Mouse.Read more (PubMed: 21452230) »
- Clark SDet al. Anatomical characterization of the neuropeptide S system in the mouse brain by in situ hybridization and immunohistochemistry. J Comp Neurol 519:1867-93 (2011). Mouse.Read more (PubMed: 21452235) »
See all 2 publications for this product
Publishing research using ab18252? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"