Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

could you please , let me know what's up with my problem as soon as possible ?

as the time factor is very important for me ,

thanks ,

ANSWER:

Thank you once again for taking the time to complete our questionnaire and for your patience.

I have now heard back from our source, who have confirmed thatthey have tested Neuropilin-1 antibody on human placenta tissue, which has high expression of isoform 1 which indicates that this antibody can detect both isoforms.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been satisfactory. Reviewing the details, I am sorry there very few tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial.

I am pleased to offer you a free of charge replacement or credit note in compensation if you have purchased within the 6 month guarantee period. In order to process this, I would appreciate if you are able to provide the Abcam order reference number and date of purchase.

I can recommend you may also like to consider trying the following options:
1. antigen retrieval Try a stronger antigen retrieval eg EDTA
2. Try fresh samples.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Dear technical support team member ,

hope my email find you well, am a researcher working in china , hubie province , wuhan city , tongji medical collage .

i had bought Rabbit monoclonal anti Neuropillin 1 , 4 months ago .

my target is to detect ((( vacular endothelial cells membrane neuropillin 1)))

after looooong time using this antibody with human vascular tissue samples i can not get any positive results !!!!!

while the results was positive when using other tissue as a positive control (( human kidney tissues ))

in conclusions , this antibody can stain the neuropillin-1 in the epithelial cells but not the endothelial cells ..

so ,

please confirm that for me please ,,

and tell me if any one could use it to stain endothelial cell membrane neuropillin 1 ???

thanks and respect in advance ,

ANSWER:

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for IHC and human. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

As neuropilin 1 is expressed invascular tissue, we would expect this antibody to detect neuropilin 1 in the endothelial cells in vascular tissue. I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could also provide an image from both the tissue typeswhich would help us to assess the results. Also, if you could describe how the samples were prepared, and if there were any differences.

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.




Order Details
Antibody code:

Lot number:

Purchase order number
or preferably Abcam order number:


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, low signal, non-specific satining etc.)

Sample (Species/Tissue/Cell Type/Cell Line etc.)


Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


Permeabilization step


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)


Optimization attempts (problem solving)
How many times have you tried the IHC?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No


What steps have you altered?


Additional Notes


We would appreciate if you are also able to provide and image which woudl help us to assess the results

I would like to test an anti-neuropilin 1 antibody in zebrafish, an untested species.

ANSWER:

Thank you very much for your interest in ab71945 and ab81321.

To our knowledge, ab71945 and ab81321 have not been tested in zebrafish. Therefore, I can offer a discount off a future purchase if you buy ab71945 or ab81321now, test it inzebrafish and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab71945 or ab81321 in zebrafish. will then send a discount code. This code must be issued before purchasing ab71945 or ab81321 so please wait for my reply before ordering.

2. Purchase ab71945 or ab81321either by phone, fax, or online (www.abcam.com).

3. Test it in zebrafish.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab71945 or ab81321 turns out to be unsuitable for zebrafish, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

I am using your np1 ab81321 antibody in wb and getting a band around 130 although your data sheet says 103kDa. Indeed your sample blot does not appear to be at 103kda. Is this a typo?

ANSWER:

Thank you for contacting us. The predicted molecular weight for Neuropilin 1 is 103 kDa, calculated from the amino acid sequence. This protein has numerous glycosylations, each of which will add at least 2 kDa to the observed molecular weight. For this reason, the protein may appear in the range of 120-140 kDa.

For more information about the glycosylation sites or amino acid sequence, please see the UniProt database entry:

http://www.uniprot.org/uniprot/O14786

I hope this helps, please let me know if you need any additional information.

I work on NKT cell and recent paper showed nrp1 has some roles in this population. So, I am interested in your product anti-nrp1 monoclonal antibody (ab81321), but not sure for its applicability on FACS. Would you like to provide us a few test dose for this product, then I can purchase it after I confirm its expression?

ANSWER:

Unfortunately we do not have samples of our antibodies to distribute to customers. However, we do guarantee our antibodies to work as we state on the datasheet. We are always happy to help our customers troubleshoot antibody issues. If for any reason you are experiencing problems with an antibody within the first 6 months of your purchase and we are unable to help you troubleshoot them we will be happy to provide you with a free of charge replacement, refund or credit.

We have tested this antibody in flow cytometry with mouse, human and rat samples and know the antibody to be working well. I would encourage you to purchase this antibody and use it in your flow cytometry experiments.