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Western blot - Neuropilin 1 antibody [EPR3113] (ab81321)
![Western blot - Neuropilin 1 antibody [EPR3113] (ab81321)](/ps/datasheet/images/81/ab81321/Neuropilin-1-Primary-antibodies-ab81321-2.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neuropilin 1 antibody [EPR3113] (ab81321)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neuropilin 1 antibody [EPR3113] (ab81321)](/ps/datasheet/images/81/ab81321/Neuropilin-1-Primary-antibodies-ab81321-3.jpg)
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Product Name
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Neuropilin 1 antibody [EPR3113]
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See all Neuropilin 1 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EPR3113] to Neuropilin 1
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Immunogen
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Synthetic peptide (Human) from the cytoplasmic region
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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Flow Cyt, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/50.
IHC-P: 1/100.
IP: 1/100.
WB: 1/1000. Predicted molecular weight: 103 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human placenta
HUVEC
HepG2 cell lysates
Mouse heart tissue
Mouse kidney tissue
Rat heart lysate
Rat kidney tissue lysate
Human kidney tissue
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Cellular localization
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Cell Membrane; Single-pass type I membrane protein. Secreted (Isoform 2).
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Research areas
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Immunology >> Adaptive Immunity >> Regulatory T Cells
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF Receptors
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Relevance
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Neuropilin 1 is a membrane-bound coreceptor to a tyrosine kinase receptor for both Vascular Endothelial Growth Factor and Semaphorin family members. Neuropilin 1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EPR3113
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 50mM Tris glycine, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Neuropilin 1 antibody [EPR3113] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Neuropilin 1 antibody [EPR3113] images, references, products related to ab81321 and other tools.
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Neuropilin 1 antibody [EPR3113] images:
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 Western blot - Neuropilin 1 antibody [EPR3113] (ab81321)
All lanes : Neuropilin 1 antibody [EPR3113] (ab81321) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : HUVEC cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : mouse heart tissue lysate
Lane 5 : mouse kidney tissue lysate
Lane 6 : rat heart tissue lysate
Lane 7 : rat kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP goat anti rabbit at 1/2000 dilution
Predicted band size : 103 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neuropilin 1 antibody [EPR3113] (ab81321)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using ab81321 at 1/100 dilution.
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Search PubMed (MEDLINE) for references to Neuropilin 1
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Neuropilin 1 antibody [EPR3113] - more information
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Customer reviews (feedback) regarding Neuropilin 1 antibody [EPR3113] |
Customer FAQs regarding Neuropilin 1 antibody [EPR3113] |
Protocols for Neuropilin 1 antibody [EPR3113] |
Price and availability of products related to Neuropilin 1 antibody [EPR3113] |
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Neuropilin 1 antibody [EPR3113] - related products:
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Neuropilin 1 antibody [EPR3113] - other tools:
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Contact Abcam with a Technical Enquiry about Neuropilin 1 antibody [EPR3113] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - Neuropilin 1 antibody [EPR3113] (ab81321)](/index.html?datasheet=81321#Neuropilin-1-Primary-antibodies-ab81321-2.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neuropilin 1 antibody [EPR3113] (ab81321)](/index.html?datasheet=81321#Neuropilin-1-Primary-antibodies-ab81321-3.jpg)
