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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF Receptors
Anti-Neuropilin 1 antibody (ab25998)
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab25998? Please let us know so that we can cite the reference in this datasheet
ab25998 has been referenced in 3 publications.
- Joseph D et al. Hormonal regulation and distinct functions of semaphorin-3B and semaphorin-3F in ovarian cancer. Mol Cancer Ther 9:499-509 (2010). WB; Human. PubMed: 20124444
- Korgaonkar SN et al. HIV-1 upregulates VEGF in podocytes. J Am Soc Nephrol 19:877-83 (2008). PubMed: 18443354
- Bondeva T et al. Advanced glycation end-products suppress neuropilin-1 expression in podocytes. Kidney Int : (2008). PubMed: 19037249
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Neuropilin 1 antibody (ab25998)
All lanes : Anti-Neuropilin 1 antibody (ab25998) at 1/500 dilution
Lane 1 : Untransfected COS-7 cells
Lane 2 : COS-7 cells transfected with HA-tagged Neuropilin 1
Predicted band size : 102 kDa
Immunocytochemistry/ Immunofluorescence-Neuropilin 1 antibody(ab25998)
ICC/IF image of ab25998 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25998, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Neuropilin 1 antibody(ab25998)
IHC image of ab25998 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25998, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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