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Anti-Neuropilin 1 antibody (ab25998)

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If your product does not perform as described on this datasheet, we will refund or replace your product...

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab25998 for help.

Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.

4 questions for ab25998

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Question 1

Tuesday 17-May-2011

Recently I have purchased NRP1 antibody from Abcam (product code : ab25998, lot no: GR1127-4)

I want to use this for Immunofluorescence study. In the datasheet it is mentioned that one can use the antibody at 5ug/ml conc for this purpose. However, I could not find anywhere the concentration of the supplied product.

ANSWER:

  

The concentration is 0.5 mg/ml. The antibody is titered to work at specific dilutions. This is why concentration is not mentioned on the datasheet. I have updated the datasheet with dilutions only to reflect this more clearly. For ICC/IF recommended dilution to start at is 1/200.

Question 2

Tuesday 17-May-2011

What about this antibody or what about neuropilin explains the 28 kDa discrepancy between the predicted molecular weight and the observed molecular weight?

ANSWER:

  

We used a transfection model using an HA-tagged human Neuropilin 1 sequence. The expressed protein ran near 130 kDa, similar to the observed molecular weight found in other studies (Proc. Natl. Acad. Sci. U.S.A. 97:2573-2578(2000)).

Question 3

Thursday 25-May-2006

What is the actual concentration of this product? It says antigen affinity purifed but doesn't give a concentration.

Thanks in advance,

ANSWER:

  

The concentration of this antibody is titrated to the dilution specified on our datasheet, so we do not otherwise provide the concentration. If you have specific questions about the titer, then let us know.

Question 4

Monday 08-May-2006

What is the concentration? abcam lot# 180589.

ANSWER:

  

Thank you for your enquiry. The concentration was not determined for this antibody as it was titrated to work at the following dilutions for these applications: ELISA 1/2000 and Western blot 1/500.

Please contact us again if you have additional questions.

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