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I would like to test an anti-neuropilin 1 antibody in zebrafish, an untested species. |
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ANSWER: |
Thank you very much for your interest in ab71945 and ab81321. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-Neuropilin 1 antibody (ab71945) at 1/1000 dilution + human brain whole cell tissue lysate (immunoprecipitated by 4 µg of ab71945) at 4mg
Predicted band size : 103 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Dot Blot:
1 mg peptide was blotted onto NC membrane
A: Neuropilin 1 (Phosphospecific Peptide at pT916)
B: Neuropilin 1 (Nonphospho PP)
C: Non-related phosphospecific PP were blotted by:
1: Rabbit anti-Neuropilin1(pT916) at 1/1000
2: ab71945 at 1/1000
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