|
|
|
|
Western blot - Neuropilin 1 antibody (ab71945)

(enlarge)
|
|
Dot Blot - Neuropilin 1 antibody (ab71945)

(enlarge)
|
|
|
|
|
Product Name
|
Neuropilin 1 antibody
|
| |
See all Neuropilin 1 antibodies (7)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to Neuropilin 1
|
|
Immunogen
|
A synthetic peptide surrounding the epitope LNTQS without phosphorylation at site Thr916.
|
|
Reacts with
|
Hu
|
|
Predicted to react
(species key)
|
Cow (Due to sequence homology)
|
|
Specificity
|
ab71945 recognizes Neuropilin 1 without phosphorylation at the site of Thr 916. It does not cross react with phospho Neuropilin 1.
|
|
Tested applications
(see key)
|
ELISA, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions ELISA: Use at a concentration of 0.01 - 0.1 µg/ml. IP: Use at a concentration of 2-5 µg/ml. WB: Use at a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 103 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Human Brain Tissue lysate.
|
|
Cellular localization
|
Cell Membrane; Single-pass type I membrane protein. Secreted (Isoform 2).
|
|
Research areas
|
Immunology >> Adaptive Immunity >> Regulatory T Cells Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF Receptors
|
|
Relevance
|
Neuropilin 1 is a membrane-bound coreceptor to a tyrosine kinase receptor for both Vascular Endothelial Growth Factor and Semaphorin family members. Neuropilin 1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window
|
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: antibody stabiliser Constituents: PBS, pH 7.2
|
|
Purification notes
|
ab71945 is purified by Site-specific Epitope Affinity Purification.
|
|
Form
|
Liquid
|
|
Concentration
|
0.500 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Neuropilin 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Neuropilin 1 antibody images, references, products related to ab71945 and other tools.
|
| |
Neuropilin 1 antibody images:
|
Western blot - Neuropilin 1 antibody (ab71945)
Neuropilin 1 antibody (ab71945) at 1/1000 dilution + human brain whole cell tissue lysate (immunoprecipitated by 4 µg of ab71945) at 4mg
Predicted band size : 103 kDa Observed band size : 130 kDa (why is the actual band size different from the predicted?)
|
| |
Dot Blot - Neuropilin 1 antibody (ab71945)
Dot Blot:
1 mg peptide was blotted onto NC membrane
A: Neuropilin 1 (Phosphospecific Peptide at pT916)
B: Neuropilin 1 (Nonphospho PP)
C: Non-related phosphospecific PP were blotted by:
1: Rabbit anti-Neuropilin1(pT916) at 1/1000
2: ab71945 at 1/1000
|
| |
| |
|
Search PubMed (MEDLINE) for references to Neuropilin 1
|
| |
Neuropilin 1 antibody - more information
|
Customer reviews (feedback) regarding Neuropilin 1 antibody |
Customer FAQs regarding Neuropilin 1 antibody |
Protocols for Neuropilin 1 antibody |
Price and availability of products related to Neuropilin 1 antibody |
| |
Neuropilin 1 antibody - related products:
|
|
|
| |
Neuropilin 1 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Neuropilin 1 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"