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Western blot - Neuropilin 1 antibody (ab71945)
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Dot Blot - Neuropilin 1 antibody (ab71945)
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Product Name
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Neuropilin 1 antibody
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See all Neuropilin 1 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Neuropilin 1
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Immunogen
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A synthetic peptide surrounding the epitope LNTQS without phosphorylation at site Thr916.
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Reacts with
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Hu
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Predicted to react
(species key)
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Cow
(Due to sequence homology)
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Specificity
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ab71945 recognizes Neuropilin 1 without phosphorylation at the site of Thr 916. It does not cross react with phospho Neuropilin 1.
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Tested applications
(see key)
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ELISA, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at a concentration of 0.01 - 0.1 µg/ml.
IP: Use at a concentration of 2-5 µg/ml.
WB: Use at a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 103 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Brain Tissue lysate.
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Cellular localization
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Cell Membrane; Single-pass type I membrane protein. Secreted (Isoform 2).
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Research areas
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Immunology >> Adaptive Immunity >> Regulatory T Cells
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF Receptors
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Relevance
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Neuropilin 1 is a membrane-bound coreceptor to a tyrosine kinase receptor for both Vascular Endothelial Growth Factor and Semaphorin family members. Neuropilin 1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: antibody stabiliser
Constituents: PBS, pH 7.2
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Purification notes
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ab71945 is purified by Site-specific Epitope Affinity Purification.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Neuropilin 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Neuropilin 1 antibody images, references, products related to ab71945 and other tools.
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Neuropilin 1 antibody images:
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 Western blot - Neuropilin 1 antibody (ab71945)
Neuropilin 1 antibody (ab71945) at 1/1000 dilution + human brain whole cell tissue lysate (immunoprecipitated by 4 µg of ab71945) at 4mg
Predicted band size : 103 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
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Dot Blot - Neuropilin 1 antibody (ab71945)
Dot Blot:
1 mg peptide was blotted onto NC membrane
A: Neuropilin 1 (Phosphospecific Peptide at pT916)
B: Neuropilin 1 (Nonphospho PP)
C: Non-related phosphospecific PP were blotted by:
1: Rabbit anti-Neuropilin1(pT916) at 1/1000
2: ab71945 at 1/1000
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Search PubMed (MEDLINE) for references to Neuropilin 1
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Neuropilin 1 antibody - more information
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Customer reviews (feedback) regarding Neuropilin 1 antibody |
Customer FAQs regarding Neuropilin 1 antibody |
Protocols for Neuropilin 1 antibody |
Price and availability of products related to Neuropilin 1 antibody |
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Neuropilin 1 antibody - related products:
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Neuropilin 1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Neuropilin 1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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