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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF Receptors
Overview
Product name
Anti-Neuropilin 1 antibody
See all Neuropilin 1 products (8) ...
Description
Rabbit polyclonal to Neuropilin 1
Specificity
ab71945 recognizes Neuropilin 1 without phosphorylation at the site of Thr 916. It does not cross react with phospho Neuropilin 1.
Tested applications
WB, IP, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Cow
Immunogen
A synthetic peptide surrounding the epitope LNTQS without phosphorylation at site Thr916.
Positive control
Human Brain Tissue lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: antibody stabiliser
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab71945 is purified by Site-specific Epitope Affinity Purification.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Adaptive Immunity >> Regulatory T Cells
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 1 µg/ml.Detects a band of approximately 130 kDa (predicted molecular weight: 103 kDa).
IP: Use a concentration of 2-5 µg/ml
ELISA: Use a concentration of 0.01 - 0.1 µg/ml.
Target
Function
The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
Tissue specificity
The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
Sequence similarities
Belongs to the neuropilin family.
Contains 2 CUB domains.
Contains 2 F5/8 type C domains.
Contains 1 MAM domain.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionO14786
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BDCA4 antibody
- CD304 antibody
- Neuropilin-1 antibody
see all
Database links
- Entrez Gene: 539369 Cow
- Entrez Gene: 8829 Human
- Omim: 602069 Human
- SwissProt: O14786 Human
- SwissProt: Q5T7F3 Human
- Unigene: 131704 Human
- Unigene: 653996 Human
Anti-Neuropilin 1 antibody images:
Western blot - Neuropilin 1 antibody (ab71945)
Anti-Neuropilin 1 antibody (ab71945) at 1/1000 dilution + human brain whole cell tissue lysate (immunoprecipitated by 4 µg of ab71945) at 4mg
Predicted band size : 103 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Dot Blot - Neuropilin 1 antibody (ab71945)
Dot Blot:
1 mg peptide was blotted onto NC membrane
A: Neuropilin 1 (Phosphospecific Peptide at pT916)
B: Neuropilin 1 (Nonphospho PP)
C: Non-related phosphospecific PP were blotted by:
1: Rabbit anti-Neuropilin1(pT916) at 1/1000
2: ab71945 at 1/1000
References for Anti-Neuropilin 1 antibody (ab71945)
ab71945 has not yet been referenced specifically in any publications.
Publishing research using ab71945? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"