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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF Receptors
Overview
Product name
Anti-Neuropilin 1 (phospho T916) antibody
See all Neuropilin 1 products (8) ...
Description
Rabbit polyclonal to Neuropilin 1 (phospho T916)
Specificity
This antibody recognizes ~130 kDa of Neuropilin 1 protein at the phosphorylation site of Thr 916. It does not react with non-phospho Neuropilin 1.
Tested applications
WB, IP, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Cow, Human
Immunogen
A synthetic peptide surrounding the epitope LNTQS with a phosphorylation site at Thr916. This sequence is identical between human and cow.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Proclin
Constituents: 1% BSA, Tris buffered saline, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Adaptive Immunity >> Regulatory T Cells
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 1 µg/ml.Predicted molecular weight: 103 kDa.
IP: Use a concentration of 2-5 µg/ml
ELISA: Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P: Use at an assay dependent dilution.
Target
Function
The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
Tissue specificity
The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
Sequence similarities
Belongs to the neuropilin family.
Contains 2 CUB domains.
Contains 2 F5/8 type C domains.
Contains 1 MAM domain.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionO14786
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BDCA4 antibody
- CD304 antibody
- Neuropilin-1 antibody
see all
Database links
- Entrez Gene: 539369 Cow
- Entrez Gene: 8829 Human
- Omim: 602069 Human
- SwissProt: O14786 Human
- SwissProt: Q5T7F3 Human
- Unigene: 131704 Human
- Unigene: 653996 Human
Anti-Neuropilin 1 (phospho T916) antibody images:
Western blot - Neuropilin 1 (phospho T916) antibody (ab71766)
Predicted band size : 103 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Lane 1: Molecular weight markers
Lane 2: Neuropilin 1 (phospho T916) antibody (ab71766) recognizing Neuropilin 1 in 4 mg protein of human brain whole tissue lysates. Protein was immunoprecipitated with 4 µg ab71766 overnight at 4 °C and probed by the same antibody at 1/1000 dilution.
Dot Blot - Neuropilin 1 (phospho T916) antibody (ab71766)
Neuropilin 1 (phospho T916) antibody (ab71766) at 1/1000 dilution recognizing Neuropilin 1 protein phosphorylated at Thr916 (Lane 1, A). Lane 2 shows a non-Abcam Neuropilin 1 antibody.
1 µg of the following peptides was blotted onto NC
membrane:
A: Neuropilin 1 (Phosphospecific peptide at pT916)
B: Neuropilin1 (Nonphospho PP)
C: Non-related phosphospecific PP
References for Anti-Neuropilin 1 (phospho T916) antibody (ab71766)
ab71766 has not yet been referenced specifically in any publications.
Publishing research using ab71766? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"