Overview

  • Product nameAnti-Neuroserpin antibodySee all Neuroserpin primary antibodies ...
  • Description
    Mouse monoclonal to Neuroserpin
  • Tested applicationsWB, IHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 17-411 of Human Neuroserpin

Properties

Applications

Our Abpromise guarantee covers the use of ab55587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSerine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator.
    • Tissue specificityPredominantly expressed in the brain.
    • Involvement in diseaseDefects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B) [MIM:604218]. FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin.
    • Sequence similaritiesBelongs to the serpin family.
    • Cellular localizationSecreted.
    • Target information above from: UniProt accession Q99574 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • DKFZp781N13156 antibody
      • Neuroserpin antibody
      • NEUS_HUMAN antibody
      • Peptidase inhibitor 12 antibody
      • PI-12 antibody
      • PI12 antibody
      • Protease inhibitor 12 antibody
      • Serine or cysteine proteinase inhibitor clade I (neuroserpin) member 1 antibody
      • Serine or cysteine proteinase inhibitor clade I member 1 antibody
      • Serpin I1 antibody
      • Serpin peptidase inhibitor clade I (neuroserpin) member 1 antibody
      • SERPINI1 antibody
      see all

    Anti-Neuroserpin antibody images

    • Neuroserpin antibody (ab55587) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human cerebral cortex.


    • Predicted band size : 46 kDa
      Neuroserpin antibody (ab55587) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-Neuroserpin antibody (ab55587)

    ab55587 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Brain)
    Specification Brain
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA
    Permeabilization No
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
    Username

    Dr. Rachel Barker

    Verified customer

    Submitted Jan 07 2010

    Application Western blot
    Sample Human Tissue lysate - whole (Brain)
    Loading amount 30 µg
    Specification Brain
    Treatment Homogenised in 1% SDS lysis buffer
    Gel Running Conditions Reduced Denaturing (4-20% Tris-HCl gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Username

    Dr. Rachel Barker

    Verified customer

    Submitted Nov 30 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"