Neurotrophin 3 Human ELISA kit (ab100615)
- Product nameNeurotrophin 3 Human ELISA kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 4 pg/ml
- Range4.12 pg/ml - 3000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 97.25 87% - 105% Serum 96.98 85% - 103% Plasma 94.45 84% - 105%
- Species reactivityReacts with: Human
- Product overview
Neurotrophin 3 (NT3) is part of the family of neurotrophic factors that control survival and differentiation of mammalian neurons. NT3 is closely related to both nerve growth factor and brain derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and affect development of neurons in the embryo when it is expressed in Human placenta.
ab100615 Neurotrophin 3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Neurotrophin 3 in serum(Neurotropin 3 concentration is low in normal serum/plasma, it may not be detected in this assay), plasma and cell culture supernatants. Optimization may be required with urine samples.
This assay employs an antibody specific for Human Neurotrophin 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Neurotrophin 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Neurotrophin 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Neurotrophin 3 bound. The Stop Solution
Optimization may be required with urine samples
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 300X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent A . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody NT-3: biotinylated anti-human NT-3 2 vials HRP-Streptavidin Concentrate 300x concentrated HRP-conjugated streptavidin 1 x 200µl NT3 Microplate (12 strips x 8 wells) coated with anti-human NT-3 1 unit NT3 Standard Human (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- MGC129711Nerve growth factor 2Neurotrophic factorNeurotrophin 3 (HDNF/NT 3)Neurotrophin 3 precursor Neurotrophin3NGF 2NGF2NT3NTF 3NTF3
Our Abpromise guarantee covers the use of ab100615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Neurotrophin 3 Human ELISA kit (ab100615)
ab100615 has not yet been referenced specifically in any publications.