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Overview

  • Product nameNeurotrophin 3 Human ELISA kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 4 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.25 87% - 105%
    Serum 96.98 85% - 103%
    Plasma 94.45 84% - 105%

  • Species reactivity
    Reacts with: Human
  • Product overview

    Neurotrophin 3 (NT3) is part of the family of neurotrophic factors that control survival and differentiation of mammalian neurons. NT3 is closely related to both nerve growth factor and brain derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and affect development of neurons in the embryo when it is expressed in Human placenta.

    ab100615 Neurotrophin 3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Neurotrophin 3 in serum(Neurotropin 3 concentration is low in normal serum/plasma, it may not be detected in this assay), plasma and cell culture supernatants. Optimization may be required with urine samples.

    This assay employs an antibody specific for Human Neurotrophin 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Neurotrophin 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Neurotrophin 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Neurotrophin 3 bound. The Stop Solution

    .

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsELISA more details

Properties

  • RelevanceNeurotrophin 3 (NT3) is part of the family of neurotrophic factors that control survival and differentiation of mammalian neurons. NT3 is closely related to both nerve growth factor and brain derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and affect development of neurons in the embryo when it is expressed in human placenta. NT3 deficient mice generated by gene targeting display severe movement defects of the limbs.
    • Alternative names
        AI316846AI835689HDNF
        MGC129711Nerve growth factor 2Neurotrophic factorNeurotrophin 3 (HDNF/NT 3)Neurotrophin 3 precursor Neurotrophin3NGF 2NGF2NT3NTF 3NTF3
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100615 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Neurotrophin 3 Human ELISA kit images

      • ELISA - Neurotrophin 3 Human ELISA kit (ab100615)
        ELISA - Neurotrophin 3 Human ELISA kit (ab100615)

      Protocols

      References for Neurotrophin 3 Human ELISA kit (ab100615)

      ab100615 has not yet been referenced specifically in any publications.

      Publishing research using ab100615 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab100615 Submit a Question for ab100615

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      To whom this may concern, I would like to request for a Certificate of Compliance for Product No. ab100615 with Lot No. GR66687. Please send the certificate to this email address. Feel free to contact me with any questions or concerns regarding this ema...

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      Thank you for contacting Abcam. Please find attached the Certificate of Compliance for ab100615 as requested. If there is anything else I can help you with, please let me kn

      I am wondering whether the NT-3 ELISA kit you offer (ab100615) is compatible with cell lysate lysed with the following buffer components: 20mM Tris-HCl pH7.4, 1mM EDTA, 1mM EGTA, 1% NP40, PI?

       Thank you for your inquiry. We haven't tested ab100615 with cell lysates.  It would most likely work, but we wouldn't be able to guarantee it since it is untested.   If you decide to test this kit with lysate samples, we recommend diluting the sample...

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      Does ab100615 cross-react with mouse? I would like to test ab75960 and ab28976 as a sandwich ELISA pair.

      Thank you very much for your interest in ab100615. This kit has not been validated for use with mouse samples, but is predicted to react based on high sequence homology. I can offer a discount off a future purchase if you buy either this ELISA ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"