Overview
- Product nameAnti-Neurotrophin 3 antibody [5C7]See all Neurotrophin 3 primary antibodies ...
- DescriptionMouse monoclonal [5C7] to Neurotrophin 3
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Human
- Immunogen
Recombinant fragment, corresponding to amino acids 139-257 of Human Neurotrophin 3 expressed in E.coli.
- Positive controlNeurotrophin 3-transfected HEK293T cell lysate; Human breast, breast adenocarcinoma, colon adenocarcinoma,kidney, kidney carcinoma, liver, liver carcinoma, lung, lung carcinoma, ovary adenocarcinoma, pancreas carcinoma, thyroid carcinoma and Human endometrium tissues.
- General notesDilute in PBS (pH7.3) before use.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA -
Concentration information loading... - PurityProtein A purified
- Purification notesab124410 is purified from Mouse ascites fluid by affinity chromatography.
- Clonality Monoclonal
- Clone number5C7
- IsotypeIgG2a
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab124410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/5000. Predicted molecular weight: 29 kDa. |
| IHC-P | IHC-P: 1/50. |
Target
- FunctionSeems to promote the survival of visceral and proprioceptive sensory neurons.
- Tissue specificityBrain and peripheral tissues.
- Sequence similaritiesBelongs to the NGF-beta family.
- Cellular localizationSecreted.
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Database links
- Entrez Gene: 4908 Human
- Omim: 162660 Human
- SwissProt: P20783 Human
- Unigene: 99171 Human
Target information above from: UniProt accession
P20783
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- HDNF antibodyMGC129711 antibodyNerve growth factor 2 antibody
- Neurotrophic factor antibodyNeurotrophin 3 precursor antibodyNeurotrophin-3 antibodyNeurotrophin3 antibodyNGF 2 antibodyNGF-2 antibodyNGF2 antibodyNT 3 antibodyNT-3 antibodyNT3 antibodyNTF 3 antibodyNTF3 antibodyNTF3_HUMAN antibody
see all
Anti-Neurotrophin 3 antibody [5C7] images
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All lanes : Anti-Neurotrophin 3 antibody [5C7] (ab124410) at 1/5000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Neurotrophin 3 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size : 29 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotrophin 3 antibody [5C7] (ab124410)ab124410 at 1/50 dilution staining Neurotrophin 3 in Paraffin-embedded Human breast tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotrophin 3 antibody [5C7] (ab124410)ab124410 at 1/50 dilution staining Neurotrophin 3 in Paraffin-embedded Human breast adenocarcinoma tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotrophin 3 antibody [5C7] (ab124410)ab124410 at 1/50 dilution staining Neurotrophin 3 in Paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotrophin 3 antibody [5C7] (ab124410)ab124410 at 1/50 dilution staining Neurotrophin 3 in Paraffin-embedded Human kidney tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotrophin 3 antibody [5C7] (ab124410)ab124410 at 1/50 dilution staining Neurotrophin 3 in Paraffin-embedded Human kidney carcinoma tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotrophin 3 antibody [5C7] (ab124410)ab124410 at 1/50 dilution staining Neurotrophin 3 in Paraffin-embedded Human liver tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotrophin 3 antibody [5C7] (ab124410)ab124410 at 1/50 dilution staining Neurotrophin 3 in Paraffin-embedded Human liver carcinoma tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotrophin 3 antibody [5C7] (ab124410)ab124410 at 1/50 dilution staining Neurotrophin 3 in Paraffin-embedded Human lung tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotrophin 3 antibody [5C7] (ab124410)ab124410 at 1/50 dilution staining Neurotrophin 3 in Paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotrophin 3 antibody [5C7] (ab124410)ab124410 at 1/50 dilution staining Neurotrophin 3 in Paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotrophin 3 antibody [5C7] (ab124410)ab124410 at 1/50 dilution staining Neurotrophin 3 in Paraffin-embedded Human pancreas carcinoma tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotrophin 3 antibody [5C7] (ab124410)ab124410 at 1/50 dilution staining Neurotrophin 3 in Paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotrophin 3 antibody [5C7] (ab124410)ab124410 at 1/50 dilution staining Neurotrophin 3 in Paraffin-embedded Human endometrium tissue by Immunohistochemistry.
References for Anti-Neurotrophin 3 antibody [5C7] (ab124410)
ab124410 has not yet been referenced specifically in any publications.
![Western blot - Anti-Neurotrophin 3 antibody [5C7] (ab124410) Western blot - Anti-Neurotrophin 3 antibody [5C7] (ab124410)](http://a.abcam.com/ps/datasheet/images/124/ab124410/Neurotrophin-3-Primary-antibodies-ab124410-1.jpg)