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Overview

  • Product nameAnti-Neurotrophin 3 antibodySee all Neurotrophin 3 primary antibodies ...
  • Description
    Rabbit polyclonal to Neurotrophin 3
  • Tested applicationsICC/IF, WB, IHC-P, IHC-Fr more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: a wide range of other species
  • Immunogen

    A synthetic peptide from mature human Neurotrophin 3 conjugated to an immunogenic carrier protein.

  • Positive controlThis antibody gave a positive result when used in the following formaldehyde fixed cell lines: SKNSH.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • PurityWhole antiserum
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab101753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/200.
WB WB: 1/300 - 1/2000. Predicted molecular weight: 29 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
IHC-P IHC-P: 1/300 - 1/2000. Antigen retrieval is recommended.
IHC-Fr IHC-Fr: 1/300 - 1/2000.

Target

  • Alternative names
      HDNF antibodyMGC129711 antibodyNerve growth factor 2 antibody
      Neurotrophic factor antibodyNeurotrophin 3 precursor antibodyNeurotrophin-3 antibodyNeurotrophin3 antibodyNGF 2 antibodyNGF-2 antibodyNGF2 antibodyNT 3 antibodyNT-3 antibodyNT3 antibodyNTF 3 antibodyNTF3 antibodyNTF3_HUMAN antibody
    see all

Anti-Neurotrophin 3 antibody images

  • ICC/IF image of ab101753 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101753 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Neurotrophin 3 antibody (ab101753)

ab101753 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"