Neurotrophin 4 Human ELISA Kit (ab100616)
- Product nameNeurotrophin 4 Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 2 pg/ml
- Range1.4 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 101.5 87% - 110% Serum 98.98 85% - 108% Plasma 95.49 83% - 107%
- Species reactivityReacts with: Human
- Product overview
The ab100616 Neurotrophin 4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Neurotrophin 4 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Neurotrophin 4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Neurotrophin 4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Neurotrophin 4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Neurotrophin 4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 700X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent A animal serum . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody NT-4: biotinylated anti-human NT-4 2 vials NT4 Microplate (12 strips x 8 wells) coated with anti-human NT-4 1 unit NT4 Standard Human (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- GLC10GLC1ONeurotrophic factor 4
- Neurotrophic factor 5Neurotrophin 5Neurotrophin 5 (neurotrophin 4/5)Neurotrophin-4Neurotrophin-5Neutrophic factor 4Neutrophic factor 5NT 4NT 4/5NT 5NT-4NT-5NT4/5 NT4P NT5NTF4NTF4_HUMANNTF5
Our Abpromise guarantee covers the use of ab100616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Neurotrophin 4 Human ELISA Kit (ab100616)
ab100616 has not yet been referenced specifically in any publications.