Neutrophil Elastase protein (Active) (ab91099)
Our Abpromise guarantee covers the use of ab91099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use at an assay dependent concentration. Ab91099 can be used as a WB positive control in conjunction with ab68672.|
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
|Functional Studies||FuncS: Use at an assay dependent dilution.|
- Bone marrow serine proteaseELA2ELANE
- Elastase 2Elastase 2 neutrophilElastase neutrophil expressedElastase-2ELNE_HUMANGEGranulocyte derived elastaseHLEHNEHuman leukocyte elastaseLeukocyte elastaseMedullasinNENeutrophil elastasePMN EPMN elastasePolymorphonuclear elastaseSCN1
Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
Contains 1 peptidase S1 domain.
Neutrophil Elastase protein (Active) images
SDS-PAGE: 12% Bis-Tris NuPAGE gel
Lane 1: 5 µg Neutrophil Elastase (reduced/heated)
Lane 2: 10 µg Neutrophil Elastase (reduced/heated)
Lane 3: 20 µg Neutrophil Elastase (reduced/heated)
Lane 4: Molecular weight markers
Anti-Neutrophil Elastase antibody (ab68672) at 1 µg/ml +
Neutrophil Elastase protein (Active) (ab91099) at 0.01 µg
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 2 minutes
References for Neutrophil Elastase protein (Active) (ab91099)
ab91099 has not yet been referenced specifically in any publications.