Anti-Nfasc186 antibody - Neuronal Marker (ab31719)
Key features and details
- Rabbit polyclonal to Nfasc186 - Neuronal Marker
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
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Overview
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Product name
Anti-Nfasc186 antibody - Neuronal Marker -
Description
Rabbit polyclonal to Nfasc186 - Neuronal Marker -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 950 - 1050 of Rat Nfasc186.
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Positive control
- Brain (Rat) Whole Tissue Lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 186 kDa (predicted molecular weight: 186 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 186 kDa (predicted molecular weight: 186 kDa). |
Target
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Relevance
Nfasc186 (Neurofascin 186) is one of two alternatively spliced products of the Neurofascin (Nfasc) gene in rat; the other being Nfasc155. The establishment of paranodal axoglial junctions in myelinated nerves where voltage-gated sodium channels are concentrated at the nodes of Ranvier is determined by myelin-forming glia. The two isoforms of Neurofascin, Nfasc186 in neurons and Nfasc155 in glia, are required for the assembly of these specialized domains. These two major Neurofascins play essential roles in assembling the nodal and paranodal domains of myelinated axons and are essential for the transition to saltatory conduction in developing vertebrate nerves. Nfasc186 (Isoform 1) is expressed at the Nodes of Ranvier while the Nfasc155 (isoforms 2 and 3) are expressed in unmyelinated axons. -
Cellular localization
Cell Membrane; Type I membrane protein. -
Database links
- Entrez Gene: 23114 Human
- Entrez Gene: 269116 Mouse
- Entrez Gene: 116690 Rat
- SwissProt: O94856 Human
- SwissProt: Q810U3 Mouse
- SwissProt: P97685 Rat
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Alternative names
- Neurofascin antibody
- NF antibody
- NF186 antibody
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Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab31719 has been referenced in 4 publications.
- Feng T et al. Protective effects of edaravone on white matter pathology in a novel mouse model of Alzheimer's disease with chronic cerebral hypoperfusion. J Cereb Blood Flow Metab 41:1437-1448 (2021). PubMed: 33106078
- Montani L et al. De novo fatty acid synthesis by Schwann cells is essential for peripheral nervous system myelination. J Cell Biol 217:1353-1368 (2018). PubMed: 29434029
- Martin PM et al. Schwannomin-interacting Protein 1 Isoform IQCJ-SCHIP1 Is a Multipartner Ankyrin- and Spectrin-binding Protein Involved in the Organization of Nodes of Ranvier. J Biol Chem 292:2441-2456 (2017). PubMed: 27979964
- Brügger V et al. HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins. PLoS Biol 13:e1002258 (2015). PubMed: 26406915