Anti-Nicastrin antibody - C-terminal (ab226299)

Overview

  • Product name
    Anti-Nicastrin antibody - C-terminal
    See all Nicastrin primary antibodies
  • Description
    Rabbit polyclonal to Nicastrin - C-terminal
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human Nicastrin aa 659-709 (C terminal). The exact sequence is proprietary. (NP_056146.1).
    Database link: Q92542

  • Positive control
    • WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab226299 was affinity purified using an epitope specific to Nicastrin immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 78 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the nicastrin family.
  • Cellular localization
    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anterior pharynx defective 2 antibody
    • APH 2 antibody
    • APH2 antibody
    • ATAG1874 antibody
    • KIAA0253 antibody
    • Ncstn antibody
    • NCT antibody
    • NICA_HUMAN antibody
    • Nicastrin antibody
    • RP11 517F10.1 antibody
    • RP11517F101 antibody
    see all

Images

  • Anti-Nicastrin antibody - C-terminal (ab226299) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate prepared using RIPA buffer at 50 µg
    Developed using the ECL technique

    Predicted band size : 78 kDa


    Exposure time : 3 minutes
  • Nicastrin was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using RIPA lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226299 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226299 at 1 µg/ml.

    Lane 1: ab226299 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 3 minutes.

References

ab226299 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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