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Synthetic peptide within Human Nicastrin aa 659-709 (C terminal). The exact sequence is proprietary. (NP_056146.1).
Database link: Q92542
Our Abpromise guarantee covers the use of ab226299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Predicted molecular weight: 78 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Nicastrin was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using RIPA lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226299 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226299 at 1 µg/ml.
Lane 1: ab226299 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
ab226299 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"